Summary
The distribution and density of the noradrenergic and acetylcholinesterase-positive nerve fibres were histochemically studied in different uterine regions of prepubertal and cycling rats in dioestrus and oestrus. Besides the rich and double innervation of blood vessels, both types of nerve fibre were found in the myometrium and cervical musculature. The non-vascular noradrenergic network looked denser at the tubal end of the horns and at the cervix, whereas the acetylcholinesterase-positive innervation was poor at the tubal end, increasing toward the cervix. Contrasting with the middle third of the uterine horn, at the tubal end, the myometrial longitudinal layer was much more innervated than the circular one, especially by the noradrenergic nerve fibres. The prepubertal rats presented an adult pattern of uterine autonomic innervation. In the cycling animals, this innervation was nearly the same during oestrus and dioestrus regarding both the density of nerve fibres and intensity of the histochemical reactions.
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References
Adham, N. & Schenk, E. A. (1969) Autonomic innervation of the rat vagina, cervix and uterus and its cyclic variation. Am. J. Obstet. Gynecol. 104, 508–16.
Alm, P., Owman, Ch., Sjöberg, N.-O., Stjernquist, M. & Sundler, F. (1986) Histochemical demonstration of a concomitant reduction in neural vasoactive intestinal polypeptide, acetylcholinesterase, and noradrenaline of cat uterus during pregnancy. Neuroscience 18, 713–26.
Bell, C. (1972) Autonomic nervous control of reproduction: circulatory and other factors. Pharmacol. Rev. 24, 657–737.
Bryman, I., Lindblom, B., Norstrom, A. & Sahni, S. (1984) Adrenoceptor mechanisms in the regulation of contractile activity in the human cervix. Obst. Gynecol. 64, 363–7.
Bulat, R., Kannan, M. S. & Garfield, R. E. (1989) Studies of the innervation of rabbit myometrium and cervix. Can. J. Physiol. Pharmacol. 67, 837–44.
Cottle, M. K. W., Cottle, W. H., Perusse, F. & Bukowiecki, L. J. (1985) An improved glyoxylic acid technique for the histochemical localization of catecholamines in brown adipose tissue. Histochem. J. 17, 1279–88.
De La Torre, J. C. (1980) An improved approach to histofluorescence using the SPG method for tissue monoamines. J. Neurosci. Meth. 3, 1–5.
Falck, B., Gardmark, S., Nybell, G., Owman, Ch., Rosengren, E. & Sjöberg, N.-O. (1974) Ovarian influence on the content of norepinephrine transmitter in guinea-pig and rat uterus. Endocrinology 94, 1475–9.
Furness, J. B. & Costa, M. (1975) The use of glyoxylic acid for the fluorescence histochemical demonstration of peripheral stores of noradrenaline and 5-hydroxytryptamine in whole mounts. Histochemistry 41, 335–52.
Garfield, R. E. (1986) Structural studies of innervation on nonpregnant rat uterus. Am. J. Physiol. 251, C41–54.
Garris, D. R., Mcconnaughey, M. M. & Saeeddar, M. (1986) Estrogen modulation of uterine adrenergic-cholinergic interaction: effects on vasoactivity and adrenergic receptors in the guinea-pig. J. Pharmacol. Exp. Ther. 239, 270–8.
Hervonen, A., Kanerva, L. & Lietzen, R. (1973) Histochemically demonstrable catecholamines and cholinesterases of the rat uterus during estrus cycle, pregnancy and after estrogen treatment. Acta Physiol. Scand. 87, 283–8.
Hollingsworth, M. (1974) The innervation of the rat cervix and its pharmacology in vitro and in vivo. Br. J. Pharmacol. 52, 539–47.
Jacobowitz, D. & Koelle, G. B. (1965) Histochemical correlations of acetylcholinesterase and catecholamines in postganglionic autonomic nerves of the cat, rabbit and guinea pig. J. Pharm. Exp. Ther. 148, 225–37.
Jordan, S. M. (1970) Adrenergic and cholinergic innervation of the reproductive tract and ovary in the guinea-pig and rabbit. J. Physiol. 210, 115P-7P.
Kanerva, L., Mustonen, T. & Teravainen, H. (1972) Histochemical studies of uterine innervation after neurectomies. Acta Physiol. Scand. 86, 359–65.
Karnovsky, M. J. & Roots, L. (1964) A ‘direct coloring’ thiocholine method for cholinesterases. J. Histochem. Cytochem. 12, 219–21.
Krall, J. F., Mori, H., Tuck, M. L., Leshon, S. L. & Korenman, S. G. (1978) Demonstration of adrenergic catecholamine receptors in rat myometrium and their regulation by sex steroid hormones. Life Sci. 23, 1073–82.
Lakomy, M., Doboszynska, T. & Szteyn, S. (1983) Adrenergic nerves in organs of the reproductive system of sexually immature pigs. Anat. Anz. 154, 149–59.
Lindvall, O. & Björklund, A. (1974) The glyoxylic acid fluorescence histochemical method: a detailed account of the methodology for the visualization of the central catecholamine neurons. Histochemistry 39, 97–127.
Long, J. A. & Evans, H. M. (1922) The oestrus cycle in the rat and associated phenomena. Mem. Univ. Calif. 6, 1–148.
Marshall, J. M. (1981) Effects of ovarian steroids and pregnancy on adrenergic nerves of uterus and oviduct. Am. J. Physiol. 240, C165–74.
Moscarini, M., Cantagalli, A., De Luca, C. & Amenta, F. (1982) Cholinergic nerves in mouse uterus. J. Neural Transm. 55, 139–48.
Mustafa, F., Fatani, J. A., El-Eikshi, H. & El-Badawi, M. G. (1987) Intrinsic innervation of the uterus in guinea pig and rat. Acta Anat. 129, 53–8.
Nakanishi, H., Mclean, J., Wood, C. & Burnstock, G. (1969) The role of sympathetic nerves in control of non-pregnant and pregnant human uterus. J. Reprod. Med. 11, 20–33.
Owman, Ch. & Sjöberg, N.-O. (1966) Adrenergic nerves in the female genital tract of the rabbit with remarks on cholinesterase-containing structures. Z. Zellf. 74, 182–97.
Owman, Ch., Rosengren, E. & Sjöberg, N.-O. (1967) Adrenergic innervation of the human female reproductive organs: a histochemical and chemical investigation. J. Obstet. Gynaecol. 30, 763–73.
Papka, R. E., Cotton, J. P. & Traurig, H. H. (1985) Comparative distribution of neuropeptide tyrosine-, vasoactive intestinal polypeptide-, substance P immunoreactive, acetylcholinesterase-positive and noradrenergic nerves in the reproductive tract of the female rat. Cell Tissue Res. 242, 475–90.
Renegar, R. H. & Rexroad, C. E. Jr. (1990) Uterine adrenergic and cholinesterase-positive nerves and myometrial catecholamine concentrations during pregnancy in sheep. Acta Anat. 137, 373–81.
Rosengren, E. & Sjöberg, N.-O. (1967) The adrenergic nerve supply to the female reproductive tract of the cat. Am. J. Anat. 121, 271–84.
Sato, S., Hayashi, R. H. & Garfield, R. E. (1989) Mechanical responses of the rat uterus, cervix, and bladder to stimulation of hypogastric and pelvic nerves in vivo. Biol. Reprod. 40, 209–19.
Sjöberg, N.-O. (1967) The adrenergic transmitter of the female reproductive tract: distribution and functional changes. Acta Physiol. Scand. (suppl) 305, 1–32.
Stjernquist, M. & Owman, Ch. (1987) Interaction of noradrenaline, NPY and VIP with the neurogenic cholinergic response of the rat uterine cervix in vitro. Acta Physiol. Scand. 131, 553–62.
Thorbert, G., Alm, P., Owman, Ch. & Sjöberg, N.-O. (1977) Regional distribution of autonomic nerves in guinea pig uterus. Am. J. Physiol. 233, C25–34.
Thorbert, G., Alm, P. & Rosengren, E. (1978) Cyclic and steroid-induced changes in adrenergic neurotransmitter level of guinea-pig uterus. Acta Obstet. Gynecol. Scand. 57, 45–8.
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Melo, R.C.N., Machado, C.R.S. Noradrenergic and acetylcholinesterase-positive nerve fibres of the uterus in sexually immature and cycling rats. Histochem J 25, 213–218 (1993). https://doi.org/10.1007/BF00163817
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DOI: https://doi.org/10.1007/BF00163817