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A new mutation protocol for obtaining auxotrophic mutants of the yeastPhaffia rhodozyma

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Biotechnology Techniques

Summary

A new mutation strategy, which involves γ-irradiation of cells followed by a selective enzymatic enrichment step, was worked out to obtain auxotrophic mutants from the astaxanthin-producing yeast P. rhodozyma. Under the optimized conditions described, different mutants suitable for strain improvement were isolated.

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References

  • Adrio, J.L., Veiga, M., Casqueiro, J., López, M. and Fernández, C. (1993). J. Gen. Appl. Microbiol. 39, 303–312.

    Google Scholar 

  • An, G.-H., Bielich, J., Auerbach, R. and Johnson, E.A. (1991). Biotechnology 9, 70–73.

    Google Scholar 

  • Chun, S.B., Chin, J.E., Bai, S. and An, G.H. (1992). FEMS Microbiol. Lett. 93, 221–226.

    Google Scholar 

  • Ferenczy, L., Sipiczki, M. and Szegedi, M. (1975). Nature 253, 46–47.

    Google Scholar 

  • Golubev, W.I. (1995) Yeast 11, 101–110.

    Google Scholar 

  • Johnson, E.A. and An, G.-H. (1991) Crit. Rev. Biotechnol. 11, 297–326.

    Google Scholar 

  • Nagy, Á., Garamszegi, N., Vágvölgyi, Cs. and Ferenczy, L. (1994). FEMS Microbiol. Lett. 123, 315–318.

    Google Scholar 

  • Nelis, H.J. and De Leenheer, A.P. (1991) J. Appl. Bacteriol. 70, 181–191.

    Google Scholar 

  • Schroeder, W.A. and Johnson, E.A. (1993) J. Gen. Microbiol. 139, 907–912.

    Google Scholar 

  • Sipiczki, M. and Ferenczy, L. (1978) Mutation Res. 50, 163–173.

    Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, Attila József University, P.O.Box 533, H-6701, Szeged, Hungary

    Zs. Palágyi, Á. Nagy, Cs. Vágvölgyi & L. Ferenczy

Authors
  1. Zs. Palágyi
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  2. Á. Nagy
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  3. Cs. Vágvölgyi
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  4. L. Ferenczy
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Palágyi, Z., Nagy, Á., Vágvölgyi, C. et al. A new mutation protocol for obtaining auxotrophic mutants of the yeastPhaffia rhodozyma . Biotechnol Tech 9, 401–402 (1995). https://doi.org/10.1007/BF00160825

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  • DOI: https://doi.org/10.1007/BF00160825

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