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Sequence analysis and structural features of the largest known protamine isolated from the sperm of the archaeogastropod Monodonta turbinata

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Abstract

Protamine of the archaeogastropod mollusc Monodonta turbinata has been isolated and characterized. With a mass of 13,476 Da, it is the largest known prolamine. Amino acid sequence of this protamine (106 residues) was established from data provided by automated sequence analysis and mass spectrometry of the protein and of its fragments. The primary structure of the NH2-terminal region exhibits repetitive sequence motifs “Basic-Ser” (mainly R-S) and both central and COOH-terminal regions are composed by arginine clusters. The amino acid sequence of Monodonta turbinata protamine shows structural similarities with other protamines from invertebrates and from birds and mammals.

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Authors and Affiliations

  1. Department d'Enginyería Química, ETSEIB, Universitat Politècnica de Catalunya, 08028, Barcelona, Spain

    M. Daban, M. Chiva & J. A. Subirana

  2. URA, CNRS 1309, Chimie des Biomolécules, Institut Pasteur, 59019, Lille, France

    A. Martinage & M. Kouach

  3. Department de Ciències Fisiològiques Humanes i de la Nutrició, Universitat de Barcelona, 08028, Spain

    M. Chiva & P. Sautière

Authors
  1. M. Daban
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  2. A. Martinage
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  3. M. Kouach
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  4. M. Chiva
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  5. J. A. Subirana
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  6. P. Sautière
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Daban, M., Martinage, A., Kouach, M. et al. Sequence analysis and structural features of the largest known protamine isolated from the sperm of the archaeogastropod Monodonta turbinata . J Mol Evol 40, 663–670 (1995). https://doi.org/10.1007/BF00160515

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  • DOI: https://doi.org/10.1007/BF00160515

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