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Transformation of the astaxanthin-producing yeast Phaffia rhodozyma

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Biotechnology Techniques

Summary

This paper describes the genetic transformation of the astaxanthin-producing yeast Phaffia rhodozyma with the cloning vector pGH-1. The plasmid replicates autonomously in this yeast, and the selection of transformants was possible by using both, the URA3 marker from Saccharomyces cerevisiae, and the kanamycin resistance (KmR) determinant from the bacterial transposon Tn903.

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References

  • Adrio, J., López, M., Casqueiro, J., Fernández, C. and Veiga, M. (1995). Curr. Genet. 27: 447–450.

    Google Scholar 

  • Adrio, J., Veiga, M., Casqueiro, J., López, M. and Fernández, C. (1993). J. Gen. Appl. Microbiol. 39: 321–330.

    Google Scholar 

  • An, G.H., Schuman, D.B. and Johnson, E.A. (1989). Appl. Environ. Microbiol. 55: 116–124.

    Google Scholar 

  • Christianson, T.W., Sikorski, R.S., Dante, M., Shero, J.H. and Hieter, P. (1992). Gene 110: 119–122.

    Google Scholar 

  • Chun, S.B., Chin, J.E., Bai, S. and An, G.H. (1992). FEMS Microbiol. Lett. 93: 221–226.

    Google Scholar 

  • Das, S., Kellerman, E. and Hollenberg, C.P. (1984). J. Bacteriol. 158: 1165–1167.

    Google Scholar 

  • Ernst, J.F. and Chan, R.K. (1985). J. Bacteriol. 163: 8–14.

    Google Scholar 

  • Hinnen, A., Hicks, J.B. and Fink, G.R. (1978). Proc. Natl. Acad. Sci. USA. 75: 1929–1933.

    Google Scholar 

  • Johnson, E.A. and An, G.H. (1991). CRC Crit. Rev. Biotechnol. 11: 297–326.

    Google Scholar 

  • Strathern, J.N. and Higgins, D. (1991). Recovery of plasmids from yeast into Escherichia coli: shuttle vectors. In: Guide to Yeast Genetics and Molecular Biology, C. Guthrie and G.R. Fink, eds. pp. 319–329. Academic Press, Inc. San Diego.

    Google Scholar 

  • Webster, T.D., and Dickson, R.C. (1983). Gene. 26: 243–252.

    Google Scholar 

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Authors and Affiliations

  1. Departamento de Microbiología, Facultad de Farmacia, Universidad de Santiago, 15706, Santiago de Compostela, Spain

    J. L. Adrio & M. Veiga

Authors
  1. J. L. Adrio
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  2. M. Veiga
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Adrio, J.L., Veiga, M. Transformation of the astaxanthin-producing yeast Phaffia rhodozyma . Biotechnol Tech 9, 509–512 (1995). https://doi.org/10.1007/BF00159567

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  • DOI: https://doi.org/10.1007/BF00159567

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