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A plate assay for the detection of organophosphonate mineralization by environmental bacteria, and its modification as an activity stain for identification of the carbon — phosphorus bond cleavage enzyme phosphonoacetate hydrolase

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Biotechnology Techniques

Summary

A replica plate screening technique,based on the acid molybdate assay for detection of phosphate,has been developed to permit the detection of microorganisms capable of mineralizing organophosphonates. The method was further adapted as the basis of an activity stain for the detection of the carbon - phosphorus bond cleavage enzyme phosphonoacetate hydrolase in PAGE gels.

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Authors and Affiliations

  1. School of Biology and Biochemistry and Questor Centre,The Queen's University of Belfast, Medical Biology Centre, 97, Lisburn Road, BT9 7BL, Belfast, Northern Ireland

    John W. McGrath & John P. Quinn

Authors
  1. John W. McGrath
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  2. John P. Quinn
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McGrath, J.W., Quinn, J.P. A plate assay for the detection of organophosphonate mineralization by environmental bacteria, and its modification as an activity stain for identification of the carbon — phosphorus bond cleavage enzyme phosphonoacetate hydrolase. Biotechnol Tech 9, 497–502 (1995). https://doi.org/10.1007/BF00159565

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  • DOI: https://doi.org/10.1007/BF00159565

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