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Effect of gelation temperature and gel-dissolving solution on cell viability and recovery of two Pseudomonas putida strains co-immobilized within calcium alginate or κ-carrageenan gel beads

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Biotechnology Techniques

Summary

Since a lethal effect of an increased temperature (42°C) on Pseudomonas putida strains PaW8 and PaW130 was demonstrated, strictly ionotropic gels such as calcium alginate or κ-carrageenan type X 0909 were used for cell co-immobilization, rather than a thermoionotropic κ-carrageenan gel. Among the variety of gel-dissolving solutions tested, only a 0.05M Na2CO3/0.02M citric acid solution was able to preserve around 100 % of the cell viability. A complete cell recovery was obtained from calcium alginate gel beads, while only 6 % of viable cells was recovered from the ionotropic κ-carrageenan gel.

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Authors and Affiliations

  1. Laboratoire de Technologie Enzymatique, URA n∘ 1442 du CNRS, France

    Denis D. G. Mater, Barbotin Jean-Noël & Thomas Daniel

  2. Laboratoire de Génétique Microbienne, Université de Technologie de Compiègne, Centre de Recherches de Royallieu, BP649, 60206, Compiègne cedex, France

    Denis D. G. Mater & Truffaut Nicole

  3. Laboratoire de Génie Cellulaire, 33 rue St.-Leu, Faculté des Sciences, Université de Picardie Jules Verne, 80039, Amiens cedex, France

    Barbotin Jean-Noël & Nava Saucedo José Edmundo

Authors
  1. Denis D. G. Mater
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  2. Barbotin Jean-Noël
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  3. Nava Saucedo José Edmundo
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  4. Truffaut Nicole
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  5. Thomas Daniel
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Mater, D.D.G., Jean-Noël, B., José Edmundo, N.S. et al. Effect of gelation temperature and gel-dissolving solution on cell viability and recovery of two Pseudomonas putida strains co-immobilized within calcium alginate or κ-carrageenan gel beads. Biotechnol Tech 9, 747–752 (1995). https://doi.org/10.1007/BF00159242

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  • DOI: https://doi.org/10.1007/BF00159242

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