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Carbohydrate antigen expression in murine embryonic stem cells and embryos. I. Lacto and neo-lacto determinants

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Summary

A comparative study of lacto- and neo-lacto series carbohydrate antigens between the undifferentiated and differentiated derivatives of the embryonic stem (ES) cell line E14 and expression in the early embryo is reported. Antibodies to neo-lacto and lacto (type 1 and 2) precursor chains and blood group antigens such as H (types 1 and 2) A, B and Lewis (Le-a, Le-b, Le-x, Le-y) were examined. Backbone lacto- and neo-lacto structures were present on undifferentiated cells, as were terminal α-gal, SSEA-1, Le-y and low levels of Le-a. On differentiation, Le-x (SSEA-1 determinant) disappeared as has been found for embryonal carcinoma (EC) cells, and other determinants became restricted to cells of particular morphology. These observations will aid determination of the status and phenotypic stability of long-term embryonic stem-cell cultures.

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Authors and Affiliations

  1. MRC Experimental Embryology and Teratology unit, St George's Hospital Medical School, Cranmer Terrace, Tooting, SW17 ORE, London, UK

    D. G. Brown

  2. Department of Cell and Structural Biology, University of Manchester, Stopford Building, Oxford Rd, M13 9PT, Manchester, UK

    V. N. Warren & S. J. Kimber

  3. Department of Pathology and Laboratory Medicine, University of Pennsylvania, 220 John Morgan Bldg, 19104, Philadelphia, PA, USA

    P. Påhlsson

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  1. D. G. Brown
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  3. P. Påhlsson
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  4. S. J. Kimber
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Brown, D.G., Warren, V.N., Påhlsson, P. et al. Carbohydrate antigen expression in murine embryonic stem cells and embryos. I. Lacto and neo-lacto determinants. Histochem J 25, 452–463 (1993). https://doi.org/10.1007/BF00157810

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