Abstract
The effects of gelling agent and abscisic acid on the morphogenetic response of seed-derived callus cultures of Poa pratensis L. were investigated. On medium solidified with Gelrite, the regeneration frequency of the calluses was twice as high as compared to agar-solidified medium. The average number of green shoots per regenerating callus was not influenced by the type of gelling agent used. When abscisic acid was added to the differentiation medium only, or to both the differentiation medium and the regeneration medium, the percentage of calluses with somatic embryos or embryo-like structures increased (up to 29.6%) as compared to the control (16.4%). The plant formation frequency, however, was not affected by abscisic acid.
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Van Ark, H.F., Zaal, M.A.C.M., Creemers-Molenaar, J. et al. Improvement of the tissue culture response of seed-derived callus cultures of Poa pratensis L.: Effect of gelling agent and abscisic acid. Plant Cell Tiss Organ Cult 27, 275–280 (1991). https://doi.org/10.1007/BF00157591
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DOI: https://doi.org/10.1007/BF00157591