Summary
An isocratic HPLC method has been developed for the single step analysis of glucose, lactate, alanine and glutamine (without prederivatization) in mammalian cell culture media. The method has been successfully connected and automated on a stirred tank reactor to monitor these components during hybridoma culture.
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References
Glacken, M. W. Biotechnology, 6 (1988), 1041–1050.
Katinger, H. Proceedings 4th European Congress on Biotechnology, 4 (1987), 21–42.
Joseph, M.H. and Mardsen, C.A. in: HPLC of small molecules: a practical approach.IRL Press (1986) 13–27.
Lin, A. and Agrawal, P. Biotechnology Letters, 10 (1988), 10, 695–698.
Mazars, C. et al. Developments in Biological Standardization, 75 (1987) 350–353.
Varecka, R., and Scheirer, W. Developments in Biological Standardization, 66 (1985), 269–272.
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Favre, E., Pugeaud, P. & Péringer, P. Automated HPLC monitoring of glucose, glutamine, lactate and alanine on suspended mammalian cell reactors. Biotechnol Tech 4, 315–320 (1990). https://doi.org/10.1007/BF00157428
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DOI: https://doi.org/10.1007/BF00157428