Abstract
Penicillin acylase from Bacillus megaterium was purified 6.22 fold using ultrafiltration followed by ammonium sulphate precipitation and gel filtration chromatography using Sephadex G-100. The final specific activity was 2.62 U/mg protein and was free from interfering proteases.
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Senthilvel, S.G., Pai, J.S. Purification of penicillin acylase of Bacillus megaterium . Biotechnol Tech 10, 611–614 (1996). https://doi.org/10.1007/BF00157371
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DOI: https://doi.org/10.1007/BF00157371