Summary
Under the control of the repressible PHO5 promoter, the expression of gene encoding interleukin 1β (Il1β) was derepressed when the medium was depleted of free inorganic phosphate (Pi). Maximum heterologous protein synthesis was obtained in the presence of 75 mg KH2PO4/1 (for 20 g glucose/l). The successful heterologous protein production greatly depends on nutritional culture conditions as Il1β production efficiency was increased by 83% through optimization of the growth medium. Comparison of different phosphate-limited cultivation strategies led to the development of a batch culture procedure with nutrient pulses to delay induced oxido-fermentative glucose metabolism and increase the Il1β production to 135 mg/l.
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Karine, B., Boutur, O., HeTene, B. et al. Influence of culture conditions on the production of heterologous interleukin 1β by Kluyveromyces lactis . Biotechnol Tech 7, 609–614 (1993). https://doi.org/10.1007/BF00156338
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DOI: https://doi.org/10.1007/BF00156338