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On-line measurement of hybridoma growth by culture fluorescence

  • Published:
Biotechnology Techniques

Summary

Fluorescence measurement of viable hybridoma cell cultures provides a convenient method for monitoring the progress of a batch culture. It is shown that cell concentration changes as low as 35,000 cells/ml during initial stages of growth can be measured reliably. This sensitivity, however, decreases to 2 × 106 cells/ml at cell concentration greater than 2 × 106 cells/ml. The culture fluorescence of hybridoma culture is a characteristic property of the cell and the medium used. Consequently, processes in which the medium composition and cell lines are invariant, a direct on-line estimate of viable cell count can be made using the method investigated in this paper.

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References

  • Armiger, W. B., Forro, J.R., Maenner, G. F. & Zabriskie, D. W. (1984). Proceedings of Biotech '84, Washington, D.C., 601–628.

  • Armiger, W.B., Forro, J.R., Montalvo, L.M., Lee, J.F. & Zabriskie, D.W. (1986). Chem. Eng. Commun., 45, 197–206.

    Google Scholar 

  • Beyeler, W., Einsele, A. & Fiechter, A. (1981). European J. Appl. Microbiol., 13, 10–14.

    Google Scholar 

  • Bosworth, J., Brimfield, A., Naylor, J. & Hunter, K. (1983). J. Immun. Meth., 62, 331–336.

    Google Scholar 

  • Duysens, L.N.M. & Amesz, J. (1957). Biochim. Biophys. Acta., 24, pp. 19–26.

    Google Scholar 

  • Harrison, D.E.F. & Chance, B. (1970). Appl. Microbiol., 19, 446–450.

    Google Scholar 

  • Henry, R. J. (1968). Clinical Chemistry: Principles and Techniques, Harper & Row, NY., 664–666.

    Google Scholar 

  • Hyvarinen, A. & Nikkila, E. (1962). Clin. Chem. Acta., 7, pp. 140.

    Google Scholar 

  • Luong, J.H.T. & Carrier, D.J. (1986). Appl. Microbiol. Biotechnol., 24, pp. 65–70.

    Google Scholar 

  • MacBride, W.R., Magee, J.A., Armiger, W.B. & Zabriskie, D.W. (1986). U.S. Pat. 4,577,110.

  • Ozato, N., Mayer, N. & Sachs, D. (1980). J. Immunol., 124, 533–540.

    Google Scholar 

  • Ristroph, D.L., Watteeuw, C.M., Armiger, W.B. & Humphrey, A.E. (1977). J. Ferment. Technol., 55, 599–608.

    Google Scholar 

  • Zabriskie, D.W., & Humphrey, A.E. (1978). App. Environ. Microbiol., 35, 337–343.

    Google Scholar 

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Authors and Affiliations

  1. Techne, Inc., 3700 Brunswick Pike, 08540, Princeton, NJ, USA

    G. MacMichael

  2. BioChem Technology, Inc., 66 Great Valley Parkway, 19355, Malvern, PA, USA

    W. B. Armiger & J. F. Lee

  3. Department of Chemical Engineering, Drexel University, 19104, Philadelphia, PA, USA

    R. Mutharasan

Authors
  1. G. MacMichael
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  2. W. B. Armiger
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  3. J. F. Lee
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  4. R. Mutharasan
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MacMichael, G., Armiger, W.B., Lee, J.F. et al. On-line measurement of hybridoma growth by culture fluorescence. Biotechnol Tech 1, 213–218 (1987). https://doi.org/10.1007/BF00155457

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  • DOI: https://doi.org/10.1007/BF00155457

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