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Permeabilization of Escherichia coli cells for enhanced penicillin acylase activity

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Biotechnology Techniques

Summary

Escherichia coli cells with penicillin acylase activity were permeabilized with aqueous solutions of the cationic detergent N-cetyl-N,N,N-trimethylammonium bromide (CTAB), at pH 8.0 and the activity was found to have almost doubled. The concentration of CTAB, the time and temperature of treatment were optimised for maximum enzyme activity and were found to be 0.2%, 20 min and 5°C respectively. Subsequently, the cell bound activity was retained for a longer period by chemical cross-linking with 0.1% glutaraldehyde.

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References

  • Balasingham K., Warburton D., Dunnill P., Lilly M.D. (1972) Biochem. Biophy. Acta 276, 250–256.

    Google Scholar 

  • Bernard J. Abbot. (1978) Advances in Applied Microbiology 20. 203–217.

    Google Scholar 

  • Bhat Nayantara., Naina N.S., Gowda L.R., Bhat S.G (1993) Enzyme Microbiol. Technol. 15. 796–800.

    Google Scholar 

  • Clarke S. (1977) Biochem.Biophy.Res. Comm. 79. 46–52.

    Google Scholar 

  • Felix, H. (1982) Anal.Biochem. 120. 211–234.

    Google Scholar 

  • Gowda L.R., Bacchawat N., Bhat S.G. (1991) Enzyme Microb. Technol. 13. 154–157.

    Google Scholar 

  • Joshi M.S., Gowda L.R., Bhat S.G. (1987) Biotechnol.Lett. 9. No. 8, 549–554.

    Google Scholar 

  • Naina N.S., Gowda L.R., Bhat S.G. (1991) Anal.Biochem. 196. 234–7

    Google Scholar 

  • Prabhune A.A., Rao B.S., Pundle A.V., Sivaraman H. (1992) Enzyme. Microb. Technol. 14. 161–163.

    Google Scholar 

  • Shewale J.G., Sivaraman H. (1989) Process Biochem. 24. 146–154. Whittacker J.J., Jackson H.J. (1980) Anal.Biochem. 105. 133–140.

    Google Scholar 

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Authors and Affiliations

  1. Department of Chemical Technology, University of Bombay, 400 019, Matunga, Bombay, India

    V. Nagalakshmi & J. S. Pai

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  1. V. Nagalakshmi
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  2. J. S. Pai
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Nagalakshmi, V., Pai, J.S. Permeabilization of Escherichia coli cells for enhanced penicillin acylase activity. Biotechnol Tech 8, 431–434 (1994). https://doi.org/10.1007/BF00154316

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