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An improved lithium acetate method for yeast transformation

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Biotechnology Techniques

Summary

We have studied the effect on Saccharomyces cerevisiae transformation frequency of varying several parameters of the lithium acetate-mediated transformation protocol first reported by Ito et al. (1983 a). We found that by sequentially optimising these conditions we obtained an approximately 50-fold increase in yield from 190 to 9000 transformants per μg of the plasmid YEp 13 for strain DBY 746.

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Author notes

  1. Christine Fleming

    Present address: Murphy and Son Ltd, Alpine St., Nottingham, UK

Authors and Affiliations

  1. Department of Bioscience and Biotechnology, University of Strathclyde, G1 1XW, Glasgow, UK

    Stewart D. Finlayson, Christine Fleming, David R. Berry & John R. Johnston

Authors
  1. Stewart D. Finlayson
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  2. Christine Fleming
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  3. David R. Berry
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  4. John R. Johnston
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Finlayson, S.D., Fleming, C., Berry, D.R. et al. An improved lithium acetate method for yeast transformation. Biotechnol Tech 5, 13–18 (1991). https://doi.org/10.1007/BF00152747

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  • DOI: https://doi.org/10.1007/BF00152747

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