Summary
A convenient and sensitive procedure which allows for the direct detection of carboxypeptidase A (CPA) and carboxypeptidase B (CPB) activities in crude fungal samples without any further purification is described. Carboxypeptidase activity is detected by generation of the fluorescent product Dansyl-Gly from Dansyl-Gly-Phe for CPA activity and from Dansyl-Gly-Arg for CPB activity using high voltage paper electrophoresis. CPA and CPB activities were shown to be present in the sapstaining fungus, Ophiostoma piceae, growing in liquid culture or in wood.
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Banerjee, S., Kaplan, H., Yagodnik, C. et al. A method for detecting carboxypeptidase activity in the sapstaining fungus, Ophiostoma piceae, growing in artificial medium or in wood. Biotechnol Tech 9, 241–246 (1995). https://doi.org/10.1007/BF00151569
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DOI: https://doi.org/10.1007/BF00151569