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Effect of fixation temperature on flow cytometric measurement of intracellular antibody content of hybridomas during batch culture

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Biotechnology Techniques

Abstract

In order to investigate the effect of fixation temperature on flow cytometric measurement of intracellular antibody content of hybridoma cells, cells in different growth stages during a batch culture were fixed and stored at 4 and -20 °C, respectively. Flow cytometric analysis indicates that both fixation temperatures can be used in monitoring the changes in intracellular antibody content of the cells during a batch culture. However, it is better to fix and store the cells at -20 °C than 4 °C with regard to preservation of intracellular antibody and storage stability.

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Authors and Affiliations

  1. Department of Biotechnology, Korea Advanced Institute of Science & Technology, Kuseong-Dong, 305-701, Yuseong-Gu, Taejun, Korea

    Gyun Min Lee, Alice S. Chuck & Bernhard O. Palsson

  2. Department of Chemical Engineering, University of Michigan, Herbert H. Dow Building, 48109, Ann Arbor, MI, USA

    Gyun Min Lee, Alice S. Chuck & Bernhard O. Palsson

Authors
  1. Gyun Min Lee
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  2. Alice S. Chuck
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  3. Bernhard O. Palsson
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Lee, G.M., Chuck, A.S. & Palsson, B.O. Effect of fixation temperature on flow cytometric measurement of intracellular antibody content of hybridomas during batch culture. Biotechnol Tech 7, 271–276 (1993). https://doi.org/10.1007/BF00150897

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