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Development of an electro-transformation system for Escherichia coli DH10B

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Biotechnology Techniques

Summary

Escherichia coli can be transformed to high efficiencies by subjecting a mixture of cells and DNA to a brief but intense electrical field. Factors that affect the transformation efficiency of E.coli strain DH10B were analysed. Optimal conditions gave an efficiency of 108 to 109 transformants/μg DNA with E.coli strains K803 and DH10B, and plasmids pB1221.23 and pBSK+. The use of ligated DNA resulted in 106 transformants/μg DNA. Detailed protocols for these systems are given.

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Authors and Affiliations

  1. Department of Genetics, The University of Leeds, LS2 9JT, Leeds, UK

    W. Sawahel, G. Sastry, C. Knight & D. Cove

Authors
  1. W. Sawahel
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  2. G. Sastry
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  3. C. Knight
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  4. D. Cove
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Sawahel, W., Sastry, G., Knight, C. et al. Development of an electro-transformation system for Escherichia coli DH10B . Biotechnol Tech 7, 261–266 (1993). https://doi.org/10.1007/BF00150895

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  • DOI: https://doi.org/10.1007/BF00150895

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