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Suppression of filamentation in recombinant Escherichia coli by amplified FtsZ activity

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Abstract

A stable plasmid pSYL107 containing the Alcaligenes eutrophus polyhydroxyalkanoate biosynthesis genes and the Escherichia coli ftsZ gene was constructed. Cell filamentation previously observed during the synthesis of poly(3-hydroxybutyric acid), PHB, could be suppressed by the amplified activity of FtsZ. In a defined medium XL1-Blue (pSYL107) accumulated twice as much PHB than XL1-Blue harboring pSYL105, which does not contain the ftsZ gene.

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Authors and Affiliations

  1. Department of Chemical Engineering and BioProcess Engineering Research Center Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, 305-701, Yusong-gu Taejon, Korea

    Sang Yup Lee

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  1. Sang Yup Lee
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Lee, S.Y. Suppression of filamentation in recombinant Escherichia coli by amplified FtsZ activity. Biotechnol Lett 16, 1247–1252 (1994). https://doi.org/10.1007/BF00149626

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  • DOI: https://doi.org/10.1007/BF00149626

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