Abstract
Extracts prepared from the salivary glands (SGE) of partially fed adult female Rhipicephalus appendiculatus ticks reduced the expression by human peripheral blood leukocytes (PBLs) of lipopolysaccharide (LPS)-stimulated cytokine mRNA. Treatment with SGE had no obvious effect on cytokine mRNA production when compared with untreated PBLs. LPS treatment induced or increased mRNA production for IFNα, IFNγ, TNF-α, IL-1α, IL-1β, IL-5, IL-6, IL-7 and IL-8. All the LPS-stimulated cytokine mRNAs were reduced when treated with a mixture of LPS and SGE. The results indicate the potential of ticks in modulating the cytokine network of their vertebrate hosts, possibly to facilitate blood feeding.
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References
Böyum, A. 1976. Isolation of lymphocytes, granulocytes and macrophages. Scand. J. Immunol. 5: 9–15.
Chomczynski, P. and Sacchi, N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156–159.
Inokuma, H., Kerlin, R.L., Kemp, D.H. and Willadsen, P. 1993. Effects of cattle tick (Boophilus microplus) infestation on the bovine immune system. Vet. Parasitol. 47: 107–111.
Kaufman, W.R. 1989. Tick-host interaction: a synthesis of current concepts. Parasitol. Today, 5: 47–56.
Kubeš, M., Fuchsberger, N., Labuda, M., Zuffová, E. and Nuttall, P.A. 1994. Salivary gland extracts of partially fed Dermacentor reticulatus ticks decrease natural killer cell activity in vitro. Immunology 84: 113–116.
Nuttall, P.A., Jones, L.D., Labuda, M. and Kaufman, W.R. 1994. Adaptations of arboviruses to ticks. J. Med. Entomol. 31: 1–9.
Ramachandra, R.N. and Wikel, S.K. 1992. Modulation of host-immune responses by ticks (Acari: Ixodidae): effect of salivary gland extracts on host macrophages and lymphocyte cytokine production. J. Med. Entomol. 29: 818–826.
Ribeiro, J.M.C., Makoul, G.T., Levine, J., Robinson, D.R. and Spielman, A. 1985. Antihaemostatic, anti-inflammatory, and immunosuppressive properties of the saliva of a tick, Ixodes dammini. J. Exp. Med. 161: 332–344.
Sonenshine, D.E. 1991. Biology of Ticks, Vol. 1. Oxford University Press, Oxford.
Titus, R.G. and Ribeiro, J.M.C. 1990. The role of vector saliva in transmission of arthropod-borne disease. Parasitol. Today 6: 157–160.
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Fuchsberger, N., Kita, M., Hajnicka, V. et al. Ixodid tick salivary gland extracts inhibit production of lipopolysaccharide-induced mRNA of several different human cytokines. Exp Appl Acarol 19, 671–676 (1995). https://doi.org/10.1007/BF00145255
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DOI: https://doi.org/10.1007/BF00145255