Abstract
Diazocyclopentadiene (DACP) has been shown to be an effective reagent for the ethylene receptor. Treatment of mung bean sprouts or tobacco leaves with DACP in the light or in the dark inactivates much of the ethylene binding. In the light, inactivation seems to be permanent, while in the dark, the site becomes active again after the DACP diffuses away. The compound is 10 times more effective in the light than in the dark. DACP inhibits banana ripening indicating the physiological receptor is involved. It also overcomes the inhibitory effect of ethylene on mung bean seedling growth (Km = 0.09 µl/1 E) at low ethylene levels. At high ethylene levels, an apparent high ethylene level site becomes apparent (Km = 50 µl/1 E) and growth is inhibited.
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References
Burg SP and Burg EA (1967) Molecular requirements for biological activity. Plant Physiol 42: 144–152
Chapman OL and Hess TC (1984) Cyclopentadiene-O-oxide: Spectroscopic observation and photochemistry of a carbonyl oxide. Jour Amer Chem Soc 106: 1842–1843
Doering W von E and DePuy CH (1953) Diazocyclopentadiene. Jour Amer Chem Soc 75: 5955–5957
McElwee-White L and Dougherty DA (1984) Concerning the viability of 1,4,6,9-Spiro [4.4] nonatetrayl as a reactive intermediate: new biradical-to-biradical rearrangements. Jour Amer Chem Soc 106: 3466–3474
Ramirez F and Levy S (1958) Reaction of diazocyclopentadiene with triphenylphosphine. Jour Org Chem 23: 2036–2037
Regitz M and Liedhegener A (1967) Reaktionen aktiver methylenverbindungen mit aziden-XV Synthese von diazocyclopentadienen durch diazogruppenübertagung und einige reaktionen. Tetrahedron 23: 2701–2708
Sanders IO, Smith AR and Hall MA (1989) Ethylene metabolism in Pisum sativum L. Planta 179: 104–114
Sisler EC (1979) Measurement of ethylene binding in plant tissue. Plant Physiol 64: 538–542
Sisler EC (1990) Ethylene binding receptors—is there more than one? In: RP Pharis and SB Rood, eds. Plant Growth Substances, pp. 192–200. Berlin: Springer-Verlag
Sisler EC (1991) Ethylene-binding components in plants. In: AK Matoo and JC Suttle, eds. The Plant Hormone Ethylene, 81–99. Boca Raton: CRC Press
Sisler EC and Blankenship SM (1991a) Action of a photoaffinity label on ethylene processes in plants. Plant Physiol 96S: 158
Sisler EC and Blankenship SM (1991b) Retardation of tomato ripening by a photoaffinity label. Plant Physiol 96S: 158
Sisler EC, Blankenship SM and Guest M (1990) Competition of cyclooctenes and cyclooctadienes for ethylene binding and activity in plants. Plant Growth Reg 9: 157–164
Sisler EC and Wood C (1988) Competition of unsaturated compounds and ethylene for binding and action. Plant Growth Reg 7: 181–191
Sisler EC and Yang SF (1984) Anti-ethylene effects of cis-2-butene and cyclic olefins. Phytochem. 23: 2765–2768
Venis MA (1985) Hormone binding sites in plants. New York: Longman, 191
Warner HL and Leopold AC (1971) Timing of growth responses in peas. Biochim Biophys Res Comm 44: 989–994
Weil T and Cais M (1963) A simplified procedure for the preparation of diazocyclopentadiene and some related compounds. Jour Org Chem 28: 24–72
Young RE, Pratt HK and Biale JB (1952) Manometric determination of low concentrations of ethylene. Anal Chem 24: 511–555
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Sisler, E.C., Blankenship, S.M. Diazocyclopentadiene (DACP), a light sensitive reagent for the ethylene receptor in plants. Plant Growth Regul 12, 125–132 (1993). https://doi.org/10.1007/BF00144593
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DOI: https://doi.org/10.1007/BF00144593