Summary
Galactooligosaccharides (GalOS) production from a lactose solution (16% w/v) was studied at 70°C, pH 4.6 and 6.0 with free or immobilized β-galactosidases of Thermus aquaticus YT-1. Synthesis of OS and lactose conversion was significantly improved when using the immobilized preparation. At pH 4.6, OS accounted for approx. 35% of the total carbohydrates with a conversion rate of lactose of 80%. The general formula of OS synthesized in this way was Gal-(Gal)n-Glu with n being equal to 1 or 2 and a β anomeric configuration of the D-galactosyl moiety.
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Berger, J.L., Lee, B.H. & Lacroix, C. Oligosaccharides synthesis by free and immobilized β-galactosidases from Thermus aquaticus YT-1. Biotechnol Lett 17, 1077–1080 (1995). https://doi.org/10.1007/BF00143104
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DOI: https://doi.org/10.1007/BF00143104