Summary
An alternative approach to the regeneration of coenzymes using immobilized hydrogen dehydrogenase (hydrogenase) is described. Hydrogenase isolated from Alcaligenes Eutrophus was immobilized to porous glass particles and used in combination with alanine dehydrogenase for formation of alanine, while the NADH consumed was regenerated by molecular hydrogen. Different physical arrangements of the two enzymes were compared. Alanine was conveniently assayed with a specially designed enzyme thermistor method.
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Danielsson, B., Winqvist, F., Malpote, J.Y. et al. Regeneration of NADH with immobilized systems of alanine dehydrogenase and hydrogen dehydrogenase. Biotechnol Lett 4, 673–678 (1982). https://doi.org/10.1007/BF00141984
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DOI: https://doi.org/10.1007/BF00141984