Summary
Additon of pyruvate or leucine was found to be efficient for increasing the intracellular ratios of NADH/NAD and NADPH/NADP while reducing the coenzyme A concentration during the cultivation of Alcaligenes eutrophus. Poly-β-hydroxybutyrate (PHB) accumulation was enhanced more than 2-fold since metabolic flux of acetyl-CoA into PHB synthetic pathway could be facilitated by the changes of the cofactor concentrations.
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References
Anderson, A. J. and Dawes, E. A. (1990) Microbiol. Rev. 54, 450–472.
Braunegg, G., Sonnleitner, B., and Lafferty, R. M. (1978) Eur. J. Appi. Microbiol. Biotechnol. 6, 29–37.
Lee, I. Y., Kim, M. K., Chang, H. N., and Park, Y. H. (1994) Biotechnol. Lett. 16, 611–616.
Lee, I. Y., Kim, M. K., Chang, H. N., Park, Y. H. (1995) FEMS Microbiol. Lett. 131, 35–39.
Lee, S. Y., Lee Y. K., and Chang, H. N. (1995) J. Ferment. Bioeng. 79, 177–180.
Martin, A. and Gottschal, J. C. (1976) J. Gen. Microbiol. 94, 333–341.
Oeding, V. and Schlegel, H. G. (1973) Biochem. J. 134, 239–248.
Pruβ, B. M. and Wolfe, A. J. (1994) Molecular Microbiol. 12, 973–984.
Senior, P. J. and Dawes, E. A. (1971) Biochem. J. 125, 55–66.
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Kim, M.K., Lee, I.Y. & Park, Y.H. Metabolites and amino acids affecting cellular cofactor concentrations and Poly-β-hydroxybutyrate biosynthesis in Alcaligenes eutrophus . Biotechnol Lett 18, 559–564 (1996). https://doi.org/10.1007/BF00140203
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DOI: https://doi.org/10.1007/BF00140203