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Kinetic analysis of L-lysine production by a fluoropyruvate sensitive mutant of B.lactofermentum

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Abstract

The kinetics of L-lysine production by a fluoropyruvate sensitive (FPs) mutant of B.lactofermentum have been analysed in batch and continuous culture. Under optimal conditions (viz. T = 30° C, pH = 7.0, DO = 10 % air saturation) it was established that the initial phosphate level could be used to extend the batch culture to give an L-lysine.HCl concentration of 68.0 g/l and a yield of 0.43 g/g. In continuous culture the yield increased at low dilution rates under conditions similar to those for extended batch culture.

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Authors and Affiliations

  1. Department of Biotechnology, University of NSW, 2033, Sydney, Australia

    B. Satiawihardja, R. G. Cail & P. L. Rogers

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  1. B. Satiawihardja
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  2. R. G. Cail
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  3. P. L. Rogers
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Satiawihardja, B., Cail, R.G. & Rogers, P.L. Kinetic analysis of L-lysine production by a fluoropyruvate sensitive mutant of B.lactofermentum . Biotechnol Lett 15, 577–582 (1993). https://doi.org/10.1007/BF00138543

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