Abstract
Lines homozygous for the Adh S and Adh F alleles were extracted from a French and an African natural population of D. melanogaster. The lines from each geographic region were then crossed and the Mendelian F2 constituted the first generation subjected to selection for an increase in adult survival in the presence of 2% ethanol.
The responses to selection were similar in the two strains, in spite of their different ethanol tolerance. In less than 10 generations, life span with ethanol increased from about 7 to more than 12 days, with realized heritabilities of 0.14 and 0.18. This extension of longevity was also observed with other concentrations of ethanol but not with water alone. The increase in life span in presence of alcohol appears to result from unknown metabolic processes, which are not obligatorily related to the capacity of the flies to tolerate starvation, nor to their size, their lipid content of their ethanol tolerance. In the two lines, however, the Adh S allele was quickly eliminated, suggesting a selective advantage for the F allele.
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References
Alahiotis S. N., 1982. Adaptation of Drosophila enzymes to temperature. IV. Natural selection at the alcohol dehydrogenase locus. Genetica 59: 81–87.
Bijlsma-Meeles E. & Van Delden W., 1974. Intra- and interpopulation selection concerning the alcohol dehydrogenase locus in Drosophila melanogaster. Nature 247: 369–371.
Briscoe D. A., Robertson A. & Maipica J. M., 1975. Dominance at Adh locus in response to adult Drosophila melanogaster to environmental alcohol. Nature 255: 148–149.
Cavener D. R. & Clegg M. T., 1978. Dynamics of correlated genetic systems. IV. Multilocus effects of ethanol stress environments. Genetics 90: 629–644.
Clarke B., 1975. The contribution of ecological genetics to evolutionary theory: detecting the direct effect of natural selection on particular polymorphic loci. Genetics 79: 101–113.
Clarke B., Camfield R. G., Galvin A. M. & Pitts C. R., 1979. Environmental factors affecting the quantity of alcohol dehydrogenase in Drosophila melanogaster. Nature 280: 517–518.
David J. R., 1977. Signification d'un polymorphisme enzymatique: la deshydrogenase alcoolique chez Drosophila melanogaster. Année biol. 16: 451–472.
David J. R., 1982. Latitudinal variability of Drosophila melanogaster: allozyme frequencies divergence between European and Afrotropical populations. Biochem. Genet. 20: 747–761.
David J. R. & Bocquet C., 1975. Similarities and differences in the latitudinal adaptation of two Drosophila sibling species. Nature 257: 588–590.
David J. R., Bocquet C., Arens M. F., Fouillet P., 1976. Biological role of alcohol dehydogenase in the tolerance of Drosophila melanogaster to aliphatic alcohols: utilization of an Adh-null mutant. Biochem. Genet. 14: 989–997.
David J. R., Bocquet C., Fouillet P. & Arens M. F., 1977. Tolérance génétique à l'alcool chez Drosophila: comparaison des effects de la sélection chez D. melanogaster et D. simulans. C. r. Acad. Sci., Paris 285: 405–408.
David J. R. & Clavel M. F., 1965. Interaction entre le génotype et le milieu d'élevage. Conséquences sur les caractéristiques du développement de la Drosophile. Bull. biol. Fr. Belg. 99: 369–378.
David J. R., Fouillet P. & Arens M. F., 1974. Comparaison de la sensibilité à l'alcohol éthylique des six espèces de Drosophila du sous-groupe melanogaster. Archs Zool. exp. gén. 115: 401–410.
David J. R., Cohet Y. & Fouillet P., 1975a. Physiologie de l'inanition et utllisation des réserves chez les adultes de Drosophila melanogaster. Archs Zool. exp. gén. 116: 579–590.
David J. R., Cohet Y. & Fouillet P., 1975b. La résistance à l'inanition chez les insectes: importance de la quantité des réserves lipidiques chez les adultes de Drosophila melanogaster. C. r. Acad. Sci., Paris 280: 2571–2574.
David J. R., Van Herrewege J., Monclus M. & Prevosti A., 1979. High ethanol tolerance in two distantly related species: a probable case of recent convergent adaptation. Comp. Biochem. Physiol. 63: 53–56.
David J. R., Van Herrewege J., De Scheemaker-Louis M. & Pla E., 1981. Drosophila alcohol dehydrogenase: detoxification of isopropanol and acetone, substances not used in energy metabolism. Heredity 47: 263–268.
Day T. H., Hillier P. C. & Clarke B., 1974. Properties of genetically polymorphic isozymes of alcohol dehydrogenase in Drosophila melanogaster. Biochem. Genet. 11: 141–153.
Falconer D. S., 1960. Introduction to quantitative genetics. Oliver & Boyd, Edinburgh.
Gibson J. B., 1970. Enzyme flexibility in Drosophila melanogaster. Nature 227: 959–961.
Gibson J. B., May T. W. & Wilks A. V., 1981. Genetie variation of the alcohol dehydrogenase locus in Drosophila melanogaster in relation to environmental variation: ethanol levels in breeding sites and allozyme frequencies. Oecologia 51: 190–198.
Grell E. H., Jacobson K. B. & Murphy J. B., 1965. Alcohol dehydrogenase in Drosophila melanogaster: isozymes and genetic variants. Science 149: 80–82.
Hickey D. A. & McLean M. D., 1980. Selection for ethanol tolerance and Adh allozymes in naturol populations of Drosophila melanogaster. Genet. Res. 36: 11–15.
Holmes R. S., Moxon L. N. & Parsons P. A., 1980. Genetic variability of alcohol dehydrogenase among Australian Drosophila species: correlation of ADH biochemical phenotype with ethanol resource utilization. J. exp. Zool. 214: 199–204.
McDonald J. F., Chambers G. K., David J. R. & Ayala F. J., 1977. Adaptive response due to changes in gene regulation: a study with Drosophila. Proc. natn. Acad. Sci. U.S.A. 74: 4562–4566.
McKenzie J. A. & McKechnie S. W., 1979. A comparative study of resource utilization in natural populations of Drosophila melanogaster and D. simulans. Oecologia 40: 299–309.
McKenzie J. A. & Parsons P. A., 1972. Alcohol tolerance: an ecological parameter in the relative success of Drosophila melanogaster and D. simulans. Occologia 10: 373–388.
McKenzie J. A. & Parsons P. A., 1974. Microdifferentiation in a natural population of Drosophila melanogaster to alcohol in the environment. Genetics 77: 385–394.
Oakeshott J. G., 1976. Selection at the alcohol dehydrogenase locus in Drosophila melanogaster imposed by environmental ethanol. Genet. Res. 26: 265–274.
Oakeshott J. G., 1979. Selection affecting enzyme polymorphism in laboratory populations of Drosophila melanogaster. Oecologia 13: 341–354.
Oakeshott J. G. & Gibson J. B., 1981. Is there selection by environmental ethanol on the alcohol dehydrogenase locus in Drosophila melanogaster? In: Genetic studies of Drosophila populations, eds. J. B. Gibson & J. G. Oakeshott. Aust. Nat. Univ. Press, Canberra, pp. 103–120.
Oakeshott J. G., Gibson J. B., Anderson P. R., Knibb W. R., Anderson D. G. & Chambers G. K., 1981. Alcohol dehydrogenase and glycerol-3-phosphate dehydrogenase clines in Drosophila melanogaster on different continents. Evolution 36: 86–96.
Parsons P. A. & Stanley S. M., 1981. Comparative effects of environmental ethanol on Drosophila melanogaster and D. simulans adults, including geographic differences in D. melanogaster. In: Genetics studies of Drosophila populations, ed. by J. B. Gibson & J. G. Oakeshott. Aust. Nat. Univ. Press, Canberra, pp. 47–57.
Poulik M. D., 1957. Starch gel electrophoresis in a discontinuous system of buffer. Nature 180: 1477.
Sampsell B. & Sims S., 1982. Effect of Adh genotype and heat stress on alcohol tolerance in Drosophila melanogaster. Nature 296: 853–855.
Sang J. H., 1978. The nutritional requirements of Drosophila. In: Genetics and Biology of Drosophila, ed. by M. Ashburner & T. R. F. Wright, Vol. II. Acad. Press, N. Y., pp. 159–192.
Van Delden W., Boerema A. C. & Kamping A., 1978. The alcohol dehydrogenase polymorphism in populations of Drosophila melanogaster I. Selection in different environments. Genetics 90: 161–191.
Van Delden W., Kamping A. & Van Dijk H., 1975. Selection at the alcohol dehydrogenase locus in Drosophila melanogaster. Experientia 31: 418–419.
Van Herrewege, J., 1978. Contribution à l'étude de la nutrition de l'adulte de Drosophila melanogaster. Thèse doctorat d'Etat, Lyon, 198 pp.
Van Herrewege J. & David J. R., 1970. Reduction of longevity in Drosophila by chronic ingestion of a bacterial toxin. Expl Geront. 5: 131–141.
Van Herrewege J. & David J. R., 1974. Utilisation de l'alcool éthylique dans le métabolisme énergétique d'un insecte: influence sur la durée de survie des adultes de Drosophila melanogaster. C. r. Acad. Sci., Paris 279: 335–338.
Van Herrewege J. & David J. R., 1980. Alcohol tolerance and alcohol utilization in Drosophila: partial independence of two adaptive traits. Heredity 44: 229–235.
Vigue C. L. & Johnson F. M., 1973. Isozyme variability in species of the genus Drosophila. VI. Frequency-property-environment relationships of allelic alcohol dehydrogenase in D. melanogaster. Biochem. Genet. 9: 213–227.
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Van Herrewege, J., David, J.R. Extension of life duration by dietary ethanol in Drosophila melanogaster: response to selection in two strains of different origins. Genetica 63, 61–70 (1984). https://doi.org/10.1007/BF00137466
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DOI: https://doi.org/10.1007/BF00137466