Summary
A constitutive β-galactosidase has been localized in the cytosol of thermoacidophilic bacterium Caldariella acidophila. Cells have been entrapped in polyacrylamide gel with full retention of enzymic activity; no activity decrease is observed after 8 months of storage. Enzyme properties in entrapped cells are similar to those of the free enzyme. A 73% hydrolysis of lactose has been achieved in a continuous system on a 2 ml entrapped cell column operating at 70°C; half life in these conditions is 30 days.
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In this paper we report preliminary data on immobilization of cells of Caldariella acidophila, an extreme thermophilic bacterium having a constitutive β-galactosidase (EC 3.2.1.23) activity.
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De Rosa, M., Gambacorta, A., Nicolaus, B. et al. Immobilized bacterial cells containing a thermostable β-galactosidase. Biotechnol Lett 2, 29–34 (1980). https://doi.org/10.1007/BF00136880
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DOI: https://doi.org/10.1007/BF00136880