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Lactase production in continuous culture by Trichoderma reesei Rut-C30

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Summary

Trichoderma reesei Rut-C30 was found to produce extracellular lactase when grown on lactose medium. Maximum enzyme levels in continuous culture were observed at dilution rates (D) between 0.02 and 0.027 hr-1. The enzyme productivity reached 27.3 U/L hr at D = 0.027 hr-1. Lactase synthesis appears to be inducible and subject to catabolite repression. Optimal growth temperature and pH for enzyme production were 28°C and pH 5. Maximum enzyme activity was observed at 63°C and pH 4.6. The apparent Km, based on the nitrophenyl-galactopyranoside assay was estimated as 0.4 mM. The enzyme is suitable for lactose hydrolysis in acid whey.

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Authors and Affiliations

  1. Lab. Fermentacion, Centro De Microbiologia y Biologia Cellular, I.V.I.C., APDO. 1827, 1010A, Caracas, Venezuela

    Francisco J. Castillo

  2. Department of Chemical Engineering, University of California, 94720, Berkeley, CA, USA

    Charles R. Wilke

Authors
  1. Francisco J. Castillo
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  2. Harvey W. Blanch
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  3. Charles R. Wilke
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Castillo, F.J., Blanch, H.W. & Wilke, C.R. Lactase production in continuous culture by Trichoderma reesei Rut-C30. Biotechnol Lett 6, 593–596 (1984). https://doi.org/10.1007/BF00135688

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