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Enhancement of tryptophan production by Escherichia coli as an application of genetic engineering

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Summary

A feedback resistant trp operon plasmid that transformed a multiple mutant (trpR tnaA) of Escherichia coli was found to enhance remarkably the production of tryptophan in a bench-scale fermentor. 5.5 g of tryptophan was accumulated per litre of culture medium at 24th hr in batch. The productivity was 0.229 g/l/hr. This productivity is the highest among those ever reported by other workers. The recombinant plasmid (Tcr Trp+ I-) used was completely stable in each run when tetracycline was added by 10 μg/l into the medium.

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Authors and Affiliations

  1. Department of Fermentation Technology, Faculty of Engineering, Osaka Univ., Yamada-kami, Suita-shi, Osaka, Japan

    Shuichi Aiba, Tadayuki Imanaka & Hiroshi Tsunekawa

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  1. Shuichi Aiba
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  2. Tadayuki Imanaka
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  3. Hiroshi Tsunekawa
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Aiba, S., Imanaka, T. & Tsunekawa, H. Enhancement of tryptophan production by Escherichia coli as an application of genetic engineering. Biotechnol Lett 2, 525–530 (1980). https://doi.org/10.1007/BF00134901

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  • DOI: https://doi.org/10.1007/BF00134901

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