Summary
Equipment and methods for continuous dialysis culture are described which make feasible the continous aseptic production of dense cultures of phytoplankton (unicellular marine algae). New features include the use of independently-replaceable fibre dialysis units and renewal of the nutrient medium (sea water) by controlled hydraulic diffusion. Cell densities of up to 4 × 107 are attained.
Similar content being viewed by others
Bibliographie
Berland, B.H., Bonin, D.J. et Maestrini, S.Y. (1976). De l'emploi concomitant d'enceintes dialysantes et de tests biologiques pour la détermination des facteurs nutritionnels limitant la production primaire des eaux marines. Ann. Inst. Oceanogr. Paris 52, 45–55.
Guillard, R.R.L. et Ryther, J.H., (1962). Studies on marine planktonic diatoms I. Cyclotella nana. Hustedt and Detomela confervacea (cleve). Gran. Can. J. Microbiol. 8, 229–239.
Jensen, A., et Rystad, B. (1973). Semi-continuous monitoring of the capacity of sea water for supporting growth of phytoplankton. J. Exp. Mar. Biol. Ecol. 11, 275–285.
Jensen, A., (1976). Dialysis culture in integrated aquaculture. Dans O. Devik (éd) Harvesting polluted waters, p. 143–149. Plenum Publ. Corp., New York.
Kossut, M.G. et Maestrini, S.Y., (1977). Croissance de quelques algues unicellulaires cultivées in situ, dans des eaux oligotrophes méditerranéennes. C.R. Acad. Sci. Ser. D. 285, 393–396.
Marsot, P., Fournier, R., et Blais, C. (1981). {arfr|Culture à dialyse: emploi de fibres creuses dialysantes pour la culture massive de phytoplancton}. Can. J. Fish. Aquat. Sc. 38, 905–911.
Marsot, P. (1980). Recherche sur la mise au point de culture massive planctonique dans des enceintes dialysantes. Rapport technique #3. Pêches et Océans Canada.
Strickland, J.D.H. et Parsons, T.R. (1972). A practical handbook of seawater analysis. 2nd ed. Bull. Fish. Res. Board Can. 167, 310p.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Marsot, P. Un nouveau procede de culture continue a dialyse pour le phytoplancton. Biotechnol Lett 3, 689–694 (1981). https://doi.org/10.1007/BF00134845
Issue Date:
DOI: https://doi.org/10.1007/BF00134845