Summary
Of four organisms tested in semi-synthetic medium for the production of 2,3-butanediol from lactose, Klebsiella pneumoniae N.C.I.B. 8017 proved to be the most promising. When tested using rennet whey permeate as substrate, a butanediol concentration of 7.5 g/l, representing a yield of 0.46 g/g lactose utilized, was observed after 96 h incubation. In whey permeate where the lactose had been hydrolysed enzymatically prior to the fermentation, a butanediol concentration of 13.7 g/l, representing a yield of 0.39 g/g sugar utilized was obtained. These results indicate that lactose utilization may be a limiting step in the fermentation process.
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References
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Lee, H.K., Maddox, I.S. Microbial production of 2,3-butanediol from whey permeate. Biotechnol Lett 6, 815–818 (1984). https://doi.org/10.1007/BF00134725
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DOI: https://doi.org/10.1007/BF00134725