The present study examined the elemental distribution with depth from the surface of Bioglass 45S5 immersed in simulated body fluid (SBF) in the presence or absence of proteins derived from foetal calf serum for 960 h using energy dispersive spectroscopy (EDS). The changes in elemental composition of the surface of Bioglass were also analysed with EDS for different immersion times (10 min to 960 h) before and after immersion in SBF with or without proteins. The presence of proteins consistently altered the elemental composition of Bioglass with depth from the surface, although the development of a silica rich layer was similar in both the presence or absence of proteins. In the presence of proteins the accumulation of calcium phosphate at the surface of Bioglass was slower, formed a narrower layer and had lower concentrations of calcium and phosphate than the calcium phosphate rich layer that developed in SBF alone.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
L. HENCH, R. J. SPLINTER, W. C. ALLEN and T. K. GREENLEE, Biomed. Mater. Res. Symp. 2 (1972) 117.
L. Hench and J. Wilson, in “Silicon Biochemistry” (Wiley, Chichester, 1986 (Ciba Foundation Symposium 121)), p. 231.
T. KASUGA, K. NAKAGAWA, M. YOSHIDA, and E. MIYADE, J. Mater. Sci. 22 (1987) 3721.
T. KITSUGI, T. YAMAMURO, T. NAKAMURA, S. KOTANI, T. KOKUBO and H. TAKEUCHI, Biomaterials 14 (1993) 216.
M. JARCHO, C. H. BOLEN, M. B. THOMAS, J. BOBICK, J. F. KAY and R. H. DOREMUS, J. Mater. Sci. 11 (1976) 2027.
R. D. RAWLINGS, Clin. Mater. 14 (1993) 155.
L. HENCH, Science 208 (1980) 826.
T. KOKUBO, J. Non-Cryst. Solids 120 (1990) 138.
L. HENCH, J. Amer. Ceram. Soc. 74 (1991) 1487.
C. Y. KIM, A. E. CLARK and L. L. HENCH, J. Non-Cryst. Solids 113 (1989) 195.
T. KOKUBO, H. KUSHITANI, S. SKKA, T. KITSUGI and T. YAMAMURO, J. Biomed. Mater. Res. 24 (1990) 721.
R. E. BAIER and R. C. DUTTON, J. Biomed. Mater. Res. 3 (1981) 225.
A. L. BOSKEY, Clin. Orthop. 5 (1981) 225.
J. Mei, R. M. Shelton, M. Aindow and P. M. Marquis, In Third Euro-Ceramics 3, Proceedings of 3rd European Ceramic Society meeting, Madrid, Spain, September 1993, edited by P. DURAN and J. F. FERNANDEZ (Faebza Editrice Iberica, 1993) p. 167.
T. KOKUBO, H. KUSHITANI, C. OHTSUKI and S. SAKKA, J. Mater. Sci.: Mater. Med. 3 (1992) 79.
G. H. NANCOLLAS and S. J. ZAWACHI, Conn. Tiss. Res. 21 (1989) 239.
J. L. MEYER and H. FLEISCH, Mineral and Electrolyte Metabolism 10 (1984) 249.
R. I. MARTIN and P. W. BROWN, J. Mater. Sci.: Mater. Med. 5 (1994) 96.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mei, J., Shelton, R.M. & Marquis, P.M. Changes in the elemental composition of Bioglass during its surface development in the presence or absence of proteins. J Mater Sci: Mater Med 6, 703–707 (1995). https://doi.org/10.1007/BF00134305
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00134305