Summary
Industrial use of cellulase enzymes, including β-D-glucosidases, can be greatly improved and expanded by the discovery and characterization of more robust, thermotolerant enzymes. A new thermostable β-D-glucosidase was isolated from Acidothermus cellulolyticus culture broth. This enzyme has a molecular weight of 52.5 kDa, an isoelectric pI of 4.1, and a pH activity optimum of 4.5. The optimum temperature for activity on p-nitrophenyl-β-D-glucoside is 63°C. This value is approximately 20°C higher than temperature optima displayed by most previously characterized β-D-glucosidases.
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Adney, W.S., Tucker, M.P., Nieves, R.A. et al. Low molecular weight thermostable β-D-glucosidase from Acidothermus cellulolyticus . Biotechnol Lett 17, 49–54 (1995). https://doi.org/10.1007/BF00134195
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DOI: https://doi.org/10.1007/BF00134195