Abstract
We evaluated antibody responses to trachoma vaccine in Taiwan monkeys using the micro-immunofluorescence assay. In the experiment with B/TW-5 gradient and genetron vaccines, the level of antibody titers could be correlated with the vaccine dose administered. Immunotype-specific protection from disease or modification of disease severity was associated with higher homologous antibody titer. In the experiment with A/G17, A/SA-6, and C/TW-3 genetron vaccines, an extraordinary high antibody response was demonstrated. Again, the high homologous antibody titers were associated with complete or partial protection from eye disease caused by the eye challenge inoculations. Cross reactions between immunotype A and C in the micro-IF test were also reflected in the vaccine protection observed in this experiment. It is concluded that there is an excellent correlation between the micro-IF antibody responses, the mouse toxicity prevention test, and vaccine prevention of monkey trachoma eye disease. Study of micro-IF antibody responses should be a useful tool for development of effective trachoma vaccine.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Collier LH. Experiments with trachoma vaccines. I. Experimental system using inclusion blennorrhea virus. Lancet 1961; 1: 795–800.
Grayston JT, Wang SP, Yang YF, Woolridge RL. Trachoma virus vaccine on the course of experimental trachoma infection in blind human volunteers. J Exper Med 1962; 115: 1009–22.
Grayston JT, Woolridge RL, Wang SP, Yen CH, Yang CY, Chen KH, Chang IH. Field studies of protection from infection by experimental trachoma virus vaccine in preschool-aged children in Taiwan. Proc Soc Exper Biol Med 1963; 112: 589–95.
Grayston JT, Kim KSW, Alexander ER, Wang SP. Protective studies in monkeys with trivalent and monovalent trachoma vaccines. In: Nichols RL, ed. Trachoma and related disorders caused by chlamydia agents. Amsterdam, New York: Excerpta Medica, 1971: 377–85.
Grayston JT, Wang SP. New knowledge of chlamydiae and the diseases they cause. J Infect Dis 1975; 132: 87–105.
Grayston JT, Wang SP. The potential for vaccine against infection of genital tract with Chlamydia trachomatis (review). Sexually Transmitted Dis 1978: 5: 73–77.
Snyder JC, Nichols RL, Bell SD, Haddad NA, Murray ES, McComb DE. Vaccination against trachoma in Saudi Arabia: Design of field trials and initial results. Indust & Trop Health 1963; 6: 65–73.
Wang SP, Grayston JT. Classification of trachoma virus strains by protection of mice from toxic death. J Immunol 1963; 90: 844–56.
Wang SP, Grayston JT. Egg infectivity assay of trachoma virus. Proc Soc Exper Biol Med 1964; 115: 587–91.
Wang SP, Grayston JT. A protency test for trachoma vaccine utilizing the mouse toxicity prevention test. Am J Ophthalmol1967; 63: 1443–53.
Wang SP. Clinical evaluation of monkey eye infection with TRIC agents. Am J Ophthalmol 1967; 63: 1321–26.
Wang SP, Grayston JT, Alexander ER. Trachoma vaccine studies in monkeys. Am J Ophthalmol 1967; 63: 1615–30.
Wang SP, Grayston JT. Immunological relationship between genital TRIC, lymphogranuloma venereum and related organisms in a new microtiter indirect immunofluorescence test. Am J Ophthalmol 1970; 70: 367–74.
Wang SP. A micro-immunofluorescence method. Study of antibody response to TRIC organisms in mice. In: Michols RL, ed. Trachoma and related disorders caused by chlamydial agents. Amsterdam, New York: Excerpta Medica, 1971: 273–88.
Wang SP, Grayston JT. Micro-immunofluorescence serology of Chlamydia trachomatis. In: de la Maza LM, Peterson EM, eds. Medical virology III. Amsterdam: Eisevier Sci. Publication, 1984: 87–118.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wang, Sp., Grayston, J.T. Micro-immunofluorescence antibody responses to trachoma vaccines. Int Ophthalmol 12, 73–80 (1988). https://doi.org/10.1007/BF00133785
Issue Date:
DOI: https://doi.org/10.1007/BF00133785