Summary
Grape must and concentrated rectified grape must were used for the gluconic acid synthesis using Aspergillus niger batch cultures. The latter substrate was the better, with a production, at 72 h, of 67.43 g/l and a yield (calculated on converted glucose) of 0.96. Citric acid was also observed as a by-product. In order to decrease the residual fructose content, at the end of the gluconate production cycle, an experimental model of sequential fermentation A. niger — Rhizopus arrhizus was proposed for the synthesis of gluconic and fumaric acid. The use of Glucose-isomerase (EC 5.3.1.5) to convert fructose to glucose was also tested.
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Buzzini, P., Gobbetti, M., Rossi, J. et al. Utilization of grape must and concentrated rectified grape must to produce gluconic acid by Aspergillus niger, in batch fermentations. Biotechnol Lett 15, 151–156 (1993). https://doi.org/10.1007/BF00133015
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DOI: https://doi.org/10.1007/BF00133015