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A continuous culture study of methanol and formate utilization by the yeast Pichia pastoris

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Summary

Quantitative aspects of C1 metabolism by chemostat cultures of Pichia pastoris have been studied. Under methanol limitation the maximum growth yield was 0.41 (g/g), the maintenance energy was 9.1 mg CH3OH/g.h and the μmax was estimated to be 0.18 h−1. Formate was utilized by methanol-limited cultures, and increased the cell yield approx. 20% as compared to that on methanol alone.

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References

  • Asthana, H., Humphrey, A.E., and Moritz, V. (1971). Biotechnol. Bioeng. 13, 923–929.

    Article  CAS  Google Scholar 

  • Cooney, C.L., and Levine, D.W. (1975). In: Single Cell Protein, S.R. Tannenbaum and D.I.C. Wong, eds. pp. 402–423, Cambridge, Massachusetts and London, England: The MIT Press.

    Google Scholar 

  • Couderc, R., and Baratti, J. (1980). Agric. Biol. Chem. 44, 2279–2289.

    CAS  Google Scholar 

  • van Dijken, J.P., Otto, R., and Harder, W. (1976). Arch. Microbiol. 111, 137–144.

    Article  PubMed  Google Scholar 

  • van Dijken, J.P., Harder, W., and Quayle, J.R. (1981). In: Microbial Growth on C 1 Compounds, H. Dalton, ed. pp 191–201, London, Philadelphia, Rheine: Heyden.

    Google Scholar 

  • van Dijken, J.P., and Bos, P. (1981). Arch. Microbiol. 128, 320–324.

    Article  PubMed  Google Scholar 

  • Egli, Th., and Fiechter, A. (1981). J. Gen. Microbiol. 123, 365–369.

    CAS  Google Scholar 

  • Egli, Th. (1980). Dissertation ETH Zürich, Switzerland.

  • Geurts, Th.G.E., de Kok, H.E., and Roels, J.A. (1980). Biotechnol. Bioeng. 22, 2031–2043.

    Article  CAS  Google Scholar 

  • Goldberg, I. (1977). Process Biochem. 12 (9), 12–18.

    CAS  Google Scholar 

  • Goto, S., Okamoto, R., Kuwajima, T., and Takamatsu, A. (1976). J. Ferment. Technol. 54, 213–224.

    CAS  Google Scholar 

  • Hazeu, W., de Bruyn, J.C., and Bos, P. (1972). Arch. Microbiol. 87, 185–188.

    CAS  Google Scholar 

  • Held, W., Schlanderer, G., Reimann, J., and Dellweg, H. (1978). Europ. J. Appl. Microbiol. Biotechnol. 6, 127–132.

    Article  CAS  Google Scholar 

  • Houle, M.J., Long, D.E., and Smette, D. (1970). Anal. Letters 3, 401–409.

    CAS  Google Scholar 

  • Ivanov, V.S., and Podgorskii, V.S. (1977). Microbiol. 46, 164–170.

    Google Scholar 

  • de Kok, H.E., and Roels, J.A. (1980). Biotechnol. Bioeng. 22, 1097–1104.

    Article  Google Scholar 

  • Lang, E., and Lang. H. (1972). Z. Anal. Chem. 260, 8–10.

    Article  CAS  Google Scholar 

  • Lee, J., and Komagata, K. (1980). J. Gen. Appl. Microbiol. 26, 133–158.

    Article  CAS  Google Scholar 

  • Linton, J.D., Griffiths, K., and Gregory, M. (1981). Arch. Microbiol. 129, 119–122.

    Article  CAS  Google Scholar 

  • Ogata, K., Tani, Y., and Kato, N. (1975). In: Microbial Growth on C 1 Compounds, Society of Fermentation Technology, Japan, pp. 99–119.

    Google Scholar 

  • Papoutsakis, E., Hirt, W., and Lim, H.C. (1981). Biotechnol. Bioeng. 23, 235–242.

    Article  CAS  Google Scholar 

  • Patel, R.N., Hou, C.T., and Derelanko, P. (1983). Arch. Biochem. Biophys. 221, 135–142.

    Article  PubMed  CAS  Google Scholar 

  • Pilát, P., and Prokop, A. (1975). Biotechnol. Bioeng. 17, 1717–1728.

    Article  PubMed  Google Scholar 

  • Quayle, J.R. (1980). Biochem. Soc. Transactions 8, 1–10.

    CAS  Google Scholar 

  • Reuss, M., Gnieser, J., Reng, H.G., and Wagner, F. (1975). European J. Appl. Microbiol. 1, 295–305.

    Article  CAS  Google Scholar 

  • Roels, J.A. (1980). Biotechnol. Bioeng. 22, 33–53.

    Article  CAS  Google Scholar 

  • Swartz, J.R., and Cooney, C.L. (1981). Appl. Env. Microbiol. 41, 1206–1213.

    CAS  Google Scholar 

  • Tanaka, A., Yasuhara, S., Kawamoto, S., Fukui, S., and Osumi, M. (1976). J. Bacteriol. 126, 919–927.

    PubMed  CAS  Google Scholar 

  • Volfová, O., and Pilát, P. (1974). Folia Microbiol. 19, 249–256.

    Article  Google Scholar 

  • Volfová, O. (1980). Biotechnol. Letters 2, 369–374.

    Article  Google Scholar 

  • Waites, M.J., and Quayle, J.R. (1980). J. Gen. Microbiol. 118, 321–327.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Microbiology, Delft University of Technology, Julianalaan 67a, 2628 BC, Delft, The Netherlands

    W. Hazeu & R. A. Donker

  2. DSM Central Laboratory, PO Box 18, 6160 MD, Geleen, The Netherlands

    R. A. Donker

Authors
  1. W. Hazeu
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  2. R. A. Donker
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Hazeu, W., Donker, R.A. A continuous culture study of methanol and formate utilization by the yeast Pichia pastoris . Biotechnol Lett 5, 399–404 (1983). https://doi.org/10.1007/BF00131280

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