Summary
In shake flask growth, the presence of Vitreoscilla hemoglobin (VHb) enhanced production of recombinant α-amylase in E. coli by about 80% (generally confirming our previous results). Extension of these studies to growth in oxygen controlled fermentors showed that VHb afforded no advantage in α-amylase production when oxygen was not limiting, but resulted in about a 6-fold increase when oxygen was limiting. This increase was due almost entirely to secreted enzyme.
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Buddenhagen, R.E., Webster, D.A. & Stark, B.C. Enhancement by bacterial hemoglobin of amylase production in recombinant E. coli occurs under conditions of low O2 . Biotechnol Lett 18, 695–700 (1996). https://doi.org/10.1007/BF00130768
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DOI: https://doi.org/10.1007/BF00130768