Abstract
Paclobutrazol (PBZ)- and uniconazole (UCZ)-treated plants of Echinochloa frumentacea were shorter but had much wider leaves than untreated controls 10 days after treatment. Leaves of treated plants had a slightly higher concentration of soluble protein than the controls and exhibited enhanced activities of ascorbate peroxidase, monodehydroascorbate (MDHA) reductase, and glutathione (GSH) reductase. The triazoles did not influence the activity of dehydroascorbate (DHA) reductase. The leaves of treated plants had increased concentrations of water-soluble sulfhydryls and ascorbic acid. In contrast, the concentration of malondialdehyde (MDA), a by-product of lipid peroxidation, was lower in the leaves of treated plants than in controls. These results suggest that triazole growth regulators increased the activity of the endogenous H2O2-scavenging system in E. frumentacea.
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References
Alscher, RG (1989) Biosynthesis and antioxidant function of glutathione in plants. Physiol. Plant. 77, 457–464.
Asada, K (1984) Chloroplasts: Formation of active oxygen and its scavening. Meth. Enzymol. 105, 422–429.
Bradford, MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
Davis, TD, Steffens, GL and Sankhla, N (1988) Triazole plant growth regulators. Hortic. Rev. 10, 63–105.
Fletcher, RA and Hofstra, G (1988) Triazoles as potential plant protectants. In: D Berg and M Plempel, eds., Sterol Biosynthesis Inhibitors: Pharmaceutical and Agrochemical Aspects, 321–331, Cambridge: Ellis Horwood Ltd.
Halliwell, B (1981) Chloroplast metabolism: The structure and function of chloroplasts in green leaf cells, 179–205, Oxford: Clarendon Press.
Klaphek, S, Zimmer, J and Cosse, H (1990) Scavenging of hydrogen peroxide in the endosperm of Riccinus communis by ascorbate peroxidase. Plant Cell Physiol. 31, 1005–1013.
Larson, RA (1988) The antioxidants of higher plants. Phytochemistry 27, 969–978.
Malan, C, Greyling, MA and Gressel, J (1990) Correlation between CuZn superoxide dismutase and glutathione reductase, and environmental and xenobiotic stress tolerance in maize inbreds. Plant Sci. 69, 157–166.
Tanaka, K, Masuda, R, Sugimoto, T, Kawamura, Y and Kuboi, T (1990) An H2O2-decomposing system in cultured tobacco cells. Agric. Biol. Chem. 54, 2003–2008.
Upadhyaya, A, Davis, TD, Walser, RH, Galbraith, AB and Sankhla, N (1989) Uniconazole-induced alleviation of low temperature damage in relation to antioxidant activity. HortScience 24, 955–957.
Upadhyaya, A, Davis, TD, Larsen, MH, Walser, RH and Sankhla, N (1990) Uniconazole-induced thermotolerance in soybean seedling root tissue. Physiol. Plant. 79, 78–84.
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Sankhla, N., Upadhyaya, A., Davis, T.D. et al. Hydrogen peroxide-scavenging enzymes and antioxidants in Echinochloa frumentacea as affected by triazole growth regulators. Plant Growth Regul 11, 441–443 (1992). https://doi.org/10.1007/BF00130654
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DOI: https://doi.org/10.1007/BF00130654