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The effects of abscisic acid and methyl jasmonate on 1-aminocyclopropane 1-carboxylic acid conversion to ethylene in hypocotyl segments of sunflower seedlings, and their control by calcium and calmodulin

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Abstract

The conversion of 1-aminocyclopropane 1-carboxylic acid (ACC) to ethylene by hypocotyl segments of sunflower (Helianthus annuus L.) seedlings was inhibited by abscisic acid (ABA) and methyl jasmonate (Me-Ja), and this inhibitory effect increased with increasing concentration of both growth regulators. On the contrary, CaCl, enhanced ACC conversion to ethylene at the concentrations of 10-4 M and 5 x 10-4 M, however lower and higher concentrations had no significant action. CaCl, (5 x 10-4M) seemed to magnify the inhibition of the reaction induced by ABA, whereas it reduced (5 x 10-4M) and even abolished (10-3M) the inhibitory action of Me-Ja. The results obtained with a Ca2+ chelator (EGTA), a Ca2+ channel blocker (nifedipine) and calmodulin antagonists (W7 and TFP), given in association with ABA or Me-Ja, suggested that calcium was involved in the inhibition of ACC conversion to ethylene by ABA and Me-Ja through an interaction with calmodulin. However, the mechanism of action of the two growth regulators seemed to be different, since all treatments which resulted in a decrease in cytosolic Ca2+ concentration or in calmodulin action induced a decrease in the effect of ABA and an increase in the effect of Me-Ja.

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Abbreviations

ABA:

abscisic acid

ACC:

1-aminocyclopropane 1-carboxylic acid

EFE:

ethylene for enzyme

EGTA:

ethylene glycol-bis-2-aminoethyl tetraacetic acid

Me-Ja:

methyl jasmonate

NIF:

nifedipine

TFP:

trifluoperazine dihydrochloride

W7:

N-(6-aminohexyl)5-chloro-l-naphthalenesulfonamide hydrochloride

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Authors and Affiliations

  1. Laboratoire de Physiologie Végéale Appliquée, Université Pierre et Marie Curie, Tour 53, ler étage, 4, place Jussieu, 75252, Paris Cédex 05, France

    C. Bailly, F. Corbineau & D. Come

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  1. C. Bailly
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  2. F. Corbineau
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  3. D. Come
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Bailly, C., Corbineau, F. & Come, D. The effects of abscisic acid and methyl jasmonate on 1-aminocyclopropane 1-carboxylic acid conversion to ethylene in hypocotyl segments of sunflower seedlings, and their control by calcium and calmodulin. Plant Growth Regul 11, 349–355 (1992). https://doi.org/10.1007/BF00130641

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  • DOI: https://doi.org/10.1007/BF00130641

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