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High level expression and simple purification of recombinant human granulocyte colony-stimulating factor in E. coli

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Summary

A human granulocyte colony-stimulating factor (hG-CSF) gene was synthesized and inserted into a trp expression vector for over-expression in E. coli. A strong expression vector was constructed, and a simple purification procedure including in vitro refolding was established. The final productivity of hG-CSF was 500~600mg per l culture, and the purified hG-CSF showed the proliferation of neutrophils in vivo assays.

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Authors and Affiliations

  1. Research Laboratories of Dong-A Pharm. Co., Ltd., 449-900, Yongin-Kun, Kyunggi-Do, Korea

    Soo-Hyung Kang, Kyu-Heum Na, Jang-Hyeon Park & Choong-II Park

  2. Department of Agricultural Chemistry, Korea University, 136-075, Seoul, Korea

    Se-Yong Lee

  3. Korea Research Institute of Bioscience and Biotechnology, 305-606, Taejon, Korea

    Young-Ik Lee

Authors
  1. Soo-Hyung Kang
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  2. Kyu-Heum Na
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  3. Jang-Hyeon Park
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  4. Choong-II Park
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  5. Se-Yong Lee
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  6. Young-Ik Lee
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Kang, SH., Na, KH., Park, JH. et al. High level expression and simple purification of recombinant human granulocyte colony-stimulating factor in E. coli. Biotechnol Lett 17, 687–692 (1995). https://doi.org/10.1007/BF00130351

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  • DOI: https://doi.org/10.1007/BF00130351

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