Summary
Yeast cells (Saccharomyces cerevisiae) were immobilized in pectin gel, incubated 12 h at 30°C and then used for the continuous production of ethanol employing a wedge-shaped horizontal reactor and sugar cane molasses as the carbon source. Under steady state conditions the mean residence time was 1.6 h and the volumetric productivity 40 g EtOH/hl. The gas evolved was easily released. Successive batch incubation in a synthetic medium substantially restored the fermentative capacity of the beads already used in the continuous assay.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cysewsky, R.G., Wilke, C.R. (1978) Process design and economic studies of alternative fermentation methods for the production of ethanol. Biotechnol Bioeng 20: 1421–1444.
Dawson, P.S.S. (1977) Continuous fermentation. Annu Rep Ferment Process 1, Perlman, D. (ed), Academic Press, New York, pp 73–93
García, R., Rolz, C. (1978) Desarrollo de tecnología para producir etanol para carburante a partir de la caña de azúcar. I. Evaluación de la tecnología tradicional. ICAITI, División Investigaciones Aplicadas, Guatemala
Gencer, M.A., Mutharasan, R. (1980) Ethanol fermentation in a yeast immobilized Column fermentor. Adv Biotechnol, vol 1. Moo-Young,M., Robinson, C.W., Vezina, C. (eds) Pergamon Press, London, pp 627–633
Ghose, T.K., Tyagi R.D. (1979) Rapid ethanol fermentation of cellulose hydrolysate. I. Batch versus continuous systems. Biotechnol Bioeng 21: 1387–1400
Jackson, E.A. (1976) Brazil's national alcohol program. Process Biochem 11 (5): 29–30
Jones, R.P., Pamment, N, Greenfield, P.F. (1981) Alcohol fermentation by yeasts The effect of environmental and other variables. Process Biochem 42–49
Margaritis, A., Wilke, C.R. (1978) The rotor fermentor. II. Application to ethanol fermentation. Biotechnol Bioeng 20: 727–753
Mc Ghee, J.E., Julian, G.S.T., Detroy, R.W., Bothast, R.J. (1982) Ethanol production by immobilized Saccharomyces cerevisiae, Saccharomyces uvarun and Zymomonas mobilis. Biotechnol Bioeng 24: 1155–1163.
Moo-Young, M., Lamptey, J., Robinson, C.W. (1980) Immobilization of yeasts cells on various supports for ethanol production. Biotechnol Lett 2:541–548
Nagodawithana, T.W., Castellano, C., Steinkraus, K.H. (1974) Effect of dissolved oxygen, temperature, initial cell count and sugar concentration on the viability of Saccharomyces cerevisiae in rapid fermentations. Appl Microbiol 28 (3): 383–391.
Nagodawithana, T.W., Steinkraus, K.H. (1976) Influence of ethanol production and accumulation on the viability of Saccharomyces cevevisiae in “rapid fermentation”. Appl and Environ Microbiol 31 (2): 158–162
Navarro, A.R., Rubio, M.C., Callieri, D.A.S. (1983) Production of ethanol by yeasts immobilized in pectin. Eur J Appl Microbiol Biotechnol 17: 148–151
Navarro, J.M. (1978) Production d'ethanol par cellules adsorbees. SMT Coloque. Tolouse, France, 211–217.
Panchal, C.J., Stewart, G.G. (1980a) The effect of osmotic pressure on the production and excretion of ethanol and glycerol by a brewing yeast strain. J Inst Brew 86: 207–210
Panchal, C.J., Stewart, G.G. (1980b) Regulatory factors in alcohols fermentations. Adv in Biotechnol. In: Stewart, G.G., Russell (eds) Fifth international yeast symposium: 3–8
Panchal, C.J., Stewart, G.G. (1981) Ethanol production by a highly flocculent brewing yeast strain. Dev Ind Microbiol 22, Underkofler LA Wulf ML (eds) 711–717
Parisi, F., del Borghi, M, Ferraiolo, G. (1977) Kinetic considerations and diluting factor for alcoholic fermentation of beet molasses. Alcohol Ind Res 163–169
Pirt, S.J. (1975) Principles of microbe and cell cultivation. Blackwell Scientific Publications, Halsted Press, New York, p 223–233.
Rosario, E.J., Lee, K.J., Rogers, P.L. (1979) Kinetics of alcohol fermentation at high yeast levels. Biotechnol Bioeng 21: 1477–1482
Ryu, Y.W., Navarro, J.M., Durand, G. (1982) Comparative study of ethanol production by an immobilized yeast in a tubular reactor and in a multistage reactor. Eur J Appl Microbiol Biotechnol 15: 1–8
Shiotani, T, Yamané, T. (1981) A horizontal packed-bed bioreactor to reduce CO2 gas holdup in the continuous production of ethanol by immobilized yeast cells. Eur J Appl Microbiol Biotechnol 13: 96–101
Sitton, O.C., Foutch, G.L., Book, N.L., Gaddy, J.L. (1979) Ethanol from agricultural residues. Process Biochem 14(9): 7–10
Sitton, O.C., Gaddy, J.L. (1980) Ethanol production in an immobilized cell reactor. Biotechnol Bioeng 22: 1735–1748
Tyagi, R.D., Ghose, T.K. (1982) Studies on immobilized Saccharomyces cerevisiae. Analysis of continuous rapid ethanol fermentation in immobilized cell reactor. Biotechnol Bioeng 24: 781–795
Wada, M., Kato, J, Chibata, I. (1980) Continuous production of ethanol using immobilized growing yeast cells. Eur J Appl Microbiol Biotechnol 10: 275–287
Wick, E., Popper, K. (1977) Continuous fermentation in slant tubes. Biotechnol Bioeng 19: 235–246
Williams, D., Munnercke, D.M. (1981) The production of ethanol by immobilized yeast cells. Biotechnol Bioeng 23: 1813–1825
Author information
Authors and Affiliations
Additional information
Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del IPN, México D.F.
Member of the Scientific Researcher's Career of the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
Rights and permissions
About this article
Cite this article
Navarro, A., Marangoni, H., Plaza, I.M. et al. Horizontal reactor for the continuous production of ethanol by yeasts immobilized in pectin. Biotechnol Lett 6, 465–470 (1984). https://doi.org/10.1007/BF00129311
Issue Date:
DOI: https://doi.org/10.1007/BF00129311