Summary
The nar promoter of Escherichia coli was characterized, which is maximally induced under anaerobic conditions in the presence of nitrate. The following results were obtained; Expression of β-galactosidase was optimal at 1 % of nitrate and was not affected much by molybdate; the amount of β -galactosidase per unit volume was maximal when the nar promoter was induced at OD600 = 1.7, and when anaerobic condition was made by supplying nitrogen gas. At the optimal condition, the ratio of β-galactosidase between before and after induction was approximately 250 and Miller units were approximately 7,500. The results showed that the nar promoter can be used as an inducible promoter.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
DeBoer, H.A., Comstock, L.J., Vasser, M. (1983). Proc. Natl. Acad. Sci. U.S.A. 80, 21–25.
Goeddel, D.V., Heyneker, O.L., Hozumi, T., Arentzen, R., Itakuea, K., Yansura, D.G., Ross, M.J., Miozzari, G., Crea, R., Seeburg, P.H. (1979). Nature 281, 544–548.
Hughes, D.E., Curtis, J.E., Khosla, C., and Bailey, J.E. (1989). Biotechniques 7, 1026–1028.
Ingraham, J.L., Maaloe, O., and Neidhart, F.C. (1983) In: Growth of the Bacterial Cell. pp. 127–128, Sinauer Associates, Inc.: Massachusetts.
Iuchi, S., and Lin, E.C.C. (1987). Proc. Natl. Acad. Sci. U.S.A. 84, 3901–3905.
Khosla, C., and Bailey, J.E. (1989). J. Bacteriol. 171, 5995–6004.
Lee, S.B., Ryu, D.D.D., Siegel, R. and Park, S.H. (1988). Biotechnology and Bioengineering 31, 805–820.
Li, S.F., Rabi, T., and DeMoss, J.A. (1985). J. Bacteriol. 164, 25–32.
Li, S.F., and DeMoss, J.A. (1987). J. Bacteriol. 169, 4614–4629.
Li, S.F., and DeMoss, J.A. (1988). J. Biol. Chem. 263, 13700–13705.
Miller, J.H. (1972). In: Experiments in Molecular Genetics, 2nd ed., pp. 352–355, New York: Cold Spring Harbor Laboratory.
Remaut, E., Stanssens, P., Fiers, W. (1981). Gene 15, 81–93.
Sambrook, J., Fritch, E.F., and Maniatis, T. (1989). In: Molecular Cloning: A Laboratory Manual, 2nd ed., pp. 18.47–18.48, New York: Cold Spring Harbor Laboratory.
Siegel, R., and Ryu, D.D.D. (1985). Biotechnology and Bioengineering XXVII, 28–33.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lee, J., Cho, M., Hong, EK. et al. Characterization of the nar promoter to use as an inducible promoter. Biotechnol Lett 18, 129–134 (1996). https://doi.org/10.1007/BF00128665
Issue Date:
DOI: https://doi.org/10.1007/BF00128665