Summary
The effects of components of the medium on the production of extracellular polysaccharide (EPS) by cultured cells of Polianthes tuberosa (tuberose) were studied. Optimization of media components culturing in flask resulted in increasing EPS production from 1.4 to 4.1 g/l. In particular, relatively high concentration (10\s-5M) of 2,4-dichlorophenoxyacetic acid (2,4-D) markedly stimulated the production of EPS. Based on these results, EPS production by a 30-1 jar fermenter was attempted and the final rate of Production was 4.6 g/l at 30th day of culture. The EPS consisted mainly of acidic polysaccharides with glucuronic acid, mannose, arabinose, galactose, glucose and xylose.
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References
Akiyama Y., Eda S., Mori M., Kato K. (1984) Agric. Biol. Chem. 48: 403–407.
Albersheim P., Nevins D.J., English P.D. Karr A. (1967) Carbohydr. Res. 5: 340–345.
Aspinall G.O., Molloy J.A., Craig J.W.T. (1969) Can. J.Biochem. 47:1065–1070.
Bacic A., Moody S.F., McComb J.A., Hinch J.M., Clarke A.E. (1987) Aust. J. Plant Physiol. 14: 633–641.
Becker G.E., Hui P.A., Albersheim P. (1964) Plant Physiol. 39: 913–920.
Bitter T., Muir H.M. (1962) Anal. Biochem. 4: 330–334.
Gamborg O.L., Miller R.A., Ojima K. (1968) Exp. Cell Res. 50: 151–158.
Goto N. (1986) Physiol. Plant. 66: 49–52.
Fowler M.W., Stepan-Sarkissian G. (1985) Primary and Secondary Metabolism of Plant CellCultures, pp.66–73 Springer-Verlag, Berlin,Heidelberg.
Fujita Y., Hara Y., Ogino T., Suga C. (1981) Plant Cell Reports 1: 59–60.
Furuya T., Yoshikawa T., Orihara Y., Oda H. (1983) Planta Med. 48: 83–87.
Hale A.D., Pollock C.J., Dalton S.J. (1987) Plant Cell Reports 6: 435–438.
Hodge J.E., Hofreiter B.T. (1962) Methods inCarbohydrate Chemistry, Vol. 1, pp. 380–394 Academic Press, New York.
Linsmaier E.M., Skoog F. (1965) Physiol. Plant. 18: 100–127.
Mante S., Boll W.G. (1975) Can. J. Bot. 53: 1542–1548.
Panda A.K., Mishra S., Bisaria V.S. (1992) Biotechnol. Bioeng. 39: 1043–1051.
Parr A.J. (1989) J. Biotechnol. 10: 1–26.
Yamada Y., Endo T. (1984) Plant Cell Reports 3: 186–188.
Smith J.I., Smart N.J., Kurz W.G.W., Misawa M. (1987) Planta Med. 53: 470–474.
Takeuchi Y., Komamine A. (1978) Physiol. Plant. 42: 21–28.
White PR (1963) The Cultivation of Animal and Plant Cells, 2nd ed. pp. 59 Ponald PressCo., New York.
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Otsuji, K., Honda, Y., Sugimura, Y. et al. Production of polysaccharides in liquid cultures of Polianthes tuberosa cells. Biotechnol Lett 16, 943–948 (1994). https://doi.org/10.1007/BF00128630
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DOI: https://doi.org/10.1007/BF00128630