Summary
Bacillus alcalophilus subsp. halodurans ATCC 21591, Bacillus sp. NCIB 11203 and Bacillus sp. IMD370 are obligate alkalophiles and produce alkaline α-amylases with maxima for activity at pH 10.0. All three organisms yielded maximum amylase activity in a medium containing starch as a carbon source and yeatex as a nitrogen source with an initial pH 10.0.
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References
Bernfeld, P. (1955). Methods in Enzymology 1, 149–158.
Brock, T. D. (1978). Thermophilic microorganisms and life at high temperatures. Springer-Verlag, New York.
Fogarty, W. M. (1983). Microbial amylases. In: Microbial Enzymes and Biotechnology, W. M. Fogarty, ed. pp 1–92, Applied Science Publishers, London.
Hayashi, T., Akiba, T. and Horikoshi, K. (1988). Agric. Biol. Chem. 52 (2), 443–448.
Horikoshi, K. and Akiba, T. (1982). Alkalophilic microorganisms. A new microbial world. Japan Scientific Society Press, Tokyo, Springer-Verlag, New York.
Kelly, C. T. and Fogarty, W. M. (1976). Process Biochem. 11(6), 3–9.
Kelly, C. T., Brennan, P. A. and Fogarty, W. M. (1987). Biotechnol. Lett. 9(2), 125–130.
Kimura, T. and Horikoshi, K. (1989). Agric. Biol. Chem. 53(11), 2963–2968.
Kole, M. M. and Gerson, D. F. (1989). J. Ferment. Bioeng. 68(6), 423–427.
Kole, M. M., Draper, I. and Gerson, D. F. (1988). Appl. Microbiol. Biotechnol. 28, 404–408.
Kushner, D. J. (ed.). (1978). “Microbial Life in Extreme Environments”, Academic Press, New York.
Langworthy, T. A. (1978). Microbial life in extreme pH values. In Microbial Life in Extreme Environments (Kushner, D. J., ed.) pp 318–368. Academic Press, London and New York.
Lehninger, A. L. (1982). Principles of Biochemistry. Worth Publishers Inc., New York.
Stanier, R. Y., Adelberg, E. A. and Ingraham, J. L. (1987). General Microbiology, 5th edition, MacMillan Press, London.
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Mc Tigue, M.A., Kelly, C.T., Fogarty, W.M. et al. Production studies on the alkaline amylases of three alkalophilic Bacillus spp.. Biotechnol Lett 16, 569–574 (1994). https://doi.org/10.1007/BF00128602
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DOI: https://doi.org/10.1007/BF00128602