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Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from structurally unrelated single carbon sources by newly isolated Pseudomonas sp. EL-2

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Summary

A Pseudomonas sp. EL-2 strain capable of synthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] was isolated from activated sludge. For simulation of P(3HB-co-3HV) production in the cells, deficiency of nutrients such as NH4 +, SO4 2- and Mg2+ was crucial and the maximum content of P(3HB-co-3HV) could reach 46% on NH4 +-deficient medium. This organism synthesized P(3HB-co-3HV) with 3HV monomer in the range from 1.9 to 49.3 mol% from unrelated single carbon sources such as glucose, fructose, propionate, or sorbitol. P(3HB-co-3HV)s containing a higher fraction of 3HV were produced by adding propionic acid to glucose medium.

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Authors and Affiliations

  1. Department of Microbiology, Pusan National University, 609-735, Pusan, Korea

    Hongjoo Son & Sangjoon Lee

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  1. Hongjoo Son
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  2. Sangjoon Lee
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Son, H., Lee, S. Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from structurally unrelated single carbon sources by newly isolated Pseudomonas sp. EL-2. Biotechnol Lett 18, 1217–1222 (1996). https://doi.org/10.1007/BF00128596

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