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Metabolic burden as reflected by maintenance coefficient of recombinant Escherichia coli overexpressing target gene

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Abstract

Metabolic burden as a consequence of overexpression of target gene in a recombinant strain of E. coli 1727 has been analyzed with respect to maintenance energy coefficient (m). The values of ‘m’ for the host, uninduced recombinant and IPTG induced recombinant were determined to be 0.12, 0.17 and 0.32 g.g-1.h-1 respectively. Transient plasmid instability and nearly 33% fall in maximum specific growth rate were observed under conditions of enhanced requirements for maintenance energy.

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References

  • Bentley, W.E., Mirjalili, N., Andersen, D.C., Davis, R.H. and Kompala, D.S. (1990) Biotechnol. Bioeng. 35, 668–681.

    Google Scholar 

  • Bradford, M.M. (1976) Anal. Biochem. 72, 248–254.

    Google Scholar 

  • Dubey, A.K., Mollet, B. and Roberts, R.J. (1992) Nucl. Acids Res 20, 1579–1585.

    Google Scholar 

  • Erickson, L.E. (1979) Biotechnol. Bioeng. 21, 725–743.

    Google Scholar 

  • Lin, P.M., Lee, C.H. and Robert, R.J. (1989) Nucl. Acids Res. 17, 3001–3011.

    Google Scholar 

  • Mandel, S.J. (1960) Biotechnol. Rev. 24, 289–291.

    Google Scholar 

  • Mi, S. and Roberts, R.J. (1992) Nucl. Acids Res. 10, 4811–4816.

    Google Scholar 

  • Miller, J.H. (1972) Experiments in Molecular Genetics, Cold Spring Harbor Laboratory Press, New York.

    Google Scholar 

  • Pirt, S.J. (1975) Principles of Microbe and Cell Cultivation, Blackwell Scientific Publications, Oxford.

    Google Scholar 

  • Projan, S.J., Carleion, S. and Novick, R.P. (1983) Plasmid 9, 182–190.

    Google Scholar 

  • Stouthamer, A.H. and van Verseveld, H.W. (1987) Trends Biotechnol 5, 149–155.

    Google Scholar 

  • Sumners, D.K. (1991) Trends Biotechnol. 9, 273–278.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, 110016, Delhi Hauz Khas, New Delhi, India

    Sanjoy K Bhattacharya & Ashok K. Dubey

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  1. Sanjoy K Bhattacharya
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  2. Ashok K. Dubey
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Bhattacharya, S.K., Dubey, A.K. Metabolic burden as reflected by maintenance coefficient of recombinant Escherichia coli overexpressing target gene. Biotechnol Lett 17, 1155–1160 (1995). https://doi.org/10.1007/BF00128377

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  • DOI: https://doi.org/10.1007/BF00128377

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