Summary
Two-stage two-stream continuous ethanol fermentation using flocculating yeast was studied to produce high ethanol concentration from blackstrap cane-molasses. Optimizing pH, RQ, cell concentration, and medium molasses concentration of the first stage to give a good cell yield without a significant decrease in ethanol yield, the final ethanol concentration of as high as 92.0–93.5 g/l at an overall dilution rate of 0.10 l/h was obtained. This process could work stably as long as 3 weeks without any decreases in the efficiency.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bu'Lock, J.D., Comberbach, M.C., Ghommidh, C., and Williams, P.D. (1984). Chemistry and Industry, June, 432–434.
Comberbach, D.M., and Bu'Lock, J. D. (1984). Biotechnol. Let., 6, 129–134
Kida, K., Asano, S., Yamadaki, M., Iwasaki, K., Yamaguchi, T., and Sonoda, Y. (1990), J. Ferment. Bioeng., 69, 39–45
Kalina, V. (1987). UK Patent, GB 2 199 844 A
Kuriyama, H., Seiko, Y., Murakami, T., Kobayashi, H., and Sonoda, Y. (1985), J. Ferment. Technol., 63, 159–165
Kuriyama, H., Murakami, T., Kobayashi, H., Kida, K., and Sonoda, Y. (1988), Proc. of VIII Int. Symp. on alchohol Fuels, Nov., Tokyo, p.3–8
McNeil, B. and Kristiansen, B. (1990), Biotechnol Let., 12, 39–44
Petrik, M., Käppeli, O., and Fiechter, A. (1982), J. Gen. Microbiol., 129, 43–49
Prince, I. G. and Barford, J. P. (1982), Biotechnol. Let., 4, 263–268
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kuriyama, H., Ishibashi, H., Miyagawa, H. et al. Optimization of two-stage continuous ethanol fermentation using flocculating yeast. Biotechnol Lett 15, 415–420 (1993). https://doi.org/10.1007/BF00128287
Issue Date:
DOI: https://doi.org/10.1007/BF00128287