Summary
Several bacteria strains were choosen from pre-selected strains for further testing and characterisation. Hydrolytic activity of lipases from thermophilic bacteria was examined using olive oil as a substrate at different reaction temperatures. Alcoholytic activity was also investigated. Lipases from thermophilic bacteria have been successfully produced on a large scale. To be able to predict if these lipases can be used for transesterification reactions, these preparations need to be purified further or to be cloned.
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References
Bloomer, S. (1992) Ph.D. Thesis, Dep.of Biotechnology, Lund University, Lund, Sweden.
Christie, W.W. (1987) In “Lipid Analysis” 2nd edition. Pergamon Press, England, 53–54.
Degryse, E., Glansdorff, N. and Pierard, A. (1978) Arch. Microbiol. 117: 189–196.
Ison, A.P., Dunnill, P., and Lilly, M.D. (1990) Biocatalysis 3: 329–342.
Khor, H.T., Tan, N.H. and Chua, C.L. (1986) J. Am. Oil Chem. Soc. 63: 538–540.
Konradsdottir, M., Perttula, M., Pere, J., Viikari, L. and Kristjansson, J. (1991) System. Appl. Microbiol. 14: 190–195.
Kristjansson, J.K. and Alfredsson, G.Á. (1986) Natturufraedingurinn 56: 49–68.
Macrae, A.R. (1985) Extracellular microbial lipases In “Microbial enzymes and biotechnology” (Fogart, W.M., ed.). Applied Sci. Publisher, London, England.
Macrae, A.R. (1983) J. Am. Oil Chem. Soc. 60: 291–294.
Mukherjee, K.D. (1990) Biocatalysis, 3:277–293.
Omar, I. C., Nishio, N., Nagai, S. (1987) Agric. Biol. Chem. 51: 2153–2159.
Papaparaskevad, D., Christakopoulos, P., Kekos, D. and Macris, B.J. (1992) Biotechnol. Letters 14: 397–402.
Phillips, A. and Pretorius, H.J. (1991) Biotechnol. Letters 13: 833–838.
Sugihara, A., Shimada, Y. and Tominaga, Y. (1988) Agric. Biol. Chem. 52: 1589–1590.
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Sigurgísladóttir, S., Konráòsdóttir, M., Jónsson, Á. et al. Lipase activity of thermophilic bacteria from icelandic hot springs. Biotechnol Lett 15, 361–366 (1993). https://doi.org/10.1007/BF00128277
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DOI: https://doi.org/10.1007/BF00128277