Skip to main content
SpringerLink
Log in

The primary structure of D-amino acid oxidase from Rhodotorula gracilis

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

The amino acid sequence of D-amino acid oxidase from Rhodotorula gracilis was determined by automated Edman degradation of peptides generated by enzymatic and chemical cleavage. The enzyme monomer contains 368 amino acid residues and its sequence is homologous to that of other known D-amino acid oxidases. Six highly conserved regions appear to have a specific role in binding of coenzyme FAD, in active site topology and in peroxisomal targeting. Moreover, Rhodotorula gracilis D-amino acid oxidase contains a region with a cluster of basic amino acids, probably exposed to solvent, which is absent in other D-amino acid oxidases.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Butò, S., Pollegioni, L., D'Angiuro, L. and Pilone, M.S. (1994) Biotech. Bioeng. 44, in press

  • Curti, B., Ronchi, S. and Pilone, M.S. (1992) in: Chemistry and Biochemistry of Flavoenzymes Vol.III (F. Muller ed.), pp. 69–70, CRC Press, Boca Raton.

    Google Scholar 

  • Eggink, G., Engel, H., Vriend, G., Terpstra, P. and Witholt, B. (1990) J. Mol. Biol. 212, 135–142.

    Google Scholar 

  • Gadda, G., Negri, A. and Pilone, M.S. (1994) J. Biol. Chem. 269, 17809–17814.

    Google Scholar 

  • Lindqvist, Y. (1992) in: Chemistry and Biochemistry of Flavoenzymes Vol.III (F. Muller ed.) pp. 367–387, CRC press.

  • Yuen, S., Chui, A. H., Wilson, K. J. and Yuan, P. M. (1988) User Bulletin Applied Biosystems, 36.

  • Negri, A., Ceciliani, F., Tedeschi, G., Simonic, T. and Ronchi, S. (1992) J. Biol. Chem. 267, 11865–11871.

    Google Scholar 

  • Pilone, M. S., Pollegioni, L., Casalin, P., Curti, B. and Ronchi, S. (1989) Eur. J. Biochem. 180, 199–204.

    Google Scholar 

  • Perotti, M., Pollegioni, L. and Pilone, M.S. (1991) Eur. J. Cell Biol. 55, 104–113.

    Google Scholar 

  • Pollegioni, L., Ghisla, S. and Pilone, M. S. (1992a) Biochem. J., 286, 389–394.

    Google Scholar 

  • Pollegioni, L. and Pilone, M.S. (1992b) Prot. Expr. Purif. 3, 165–167.

    Google Scholar 

  • Pollegioni, L., Butò, S., Tischer, W., Ghisla, S. and Pilone, M. S. (1993a) Biochem. Mol. Biol. Int., 709–717.

  • Pollegioni, L., Langkau, B., Tischer, W., Ghisla, S. and Pilone, M. S. (1993b) J. Biol. Chem., 268, 13850–13857.

    Google Scholar 

  • Savidge, T. A. (1984) in: Biotechnology of Industrial Antibiotics (E.J. Vandamme ed.), pp.171–224, Dekker Inc., New York.

    Google Scholar 

  • Schrader, T. and Adreesen, R. (1993) Eur. J. Biochem. 218, 735–744.

    Google Scholar 

  • Wierenga, R. K., Tempestra, P. and Hol, W. G. J. (1986) J. Mol. Biol. 187, 101–107.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Instituto di Fisiologia Veterinaria e Biochimica, Università di Milano, Via Celoria 10, Milano, Italy

    Ludovica Faotto, Fabrizio Ceciliani & Severino Ronchi

  2. Dipartimento di Fisiologia e Biochimica Generali, Università di Milano, Via Celoria 26, Milano, Italy

    Loredano Pollegioni & Mirella S. Pilone

  3. Centro Interuniversitario per lo Studio delle Macromolecole Informazionali (CISMI), 20133, Milano, Italy

    Ludovica Faotto, Fabrizio Ceciliani & Severino Ronchi

Authors
  1. Ludovica Faotto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Loredano Pollegioni
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fabrizio Ceciliani
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Severino Ronchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mirella S. Pilone
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Faotto, L., Pollegioni, L., Ceciliani, F. et al. The primary structure of D-amino acid oxidase from Rhodotorula gracilis . Biotechnol Lett 17, 193–198 (1995). https://doi.org/10.1007/BF00127987

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00127987

Keywords

Search

Navigation