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Demonstration of D-xylose reductase and D-xylitol dehydrogenase in Pachysolen tannophilus

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Summary

The yeast, Pachysolen tannophilus, can utilize the pentose D-xylose with accumulation of significant quantities of ethanol. Cell extracts of the organism contain NADPH-linked D-xylose reductase (aldose reductase EC 1.1.1.21) and NAD-dependent D-xylitol dehydrogenase (D-xylulose reductase EC 1.1.1.9). D-Xylose was required for induction of both the D-xylitol dehydrogenase and the D-xylose reductase. Neither enzyme was found in glucose grown cell-free extracts.

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Authors and Affiliations

  1. U.S. Department of Agriculture Agricultural Research Service Northern Regional Research Center, 1815 North University Street, 61604, Peoria, Illinois, USA

    Karl L. Smiley & Paul L. Bolen

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  1. Karl L. Smiley
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  2. Paul L. Bolen
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Smiley, K.L., Bolen, P.L. Demonstration of D-xylose reductase and D-xylitol dehydrogenase in Pachysolen tannophilus . Biotechnol Lett 4, 607–610 (1982). https://doi.org/10.1007/BF00127793

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  • DOI: https://doi.org/10.1007/BF00127793

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