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Effect of triton X-100 on alkaline lipase production by Pseudomonas pseudoalcaligenes F-111

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Summary

An extracellular alkaline lipase was evaluated from Pseudomonas pseudoalcaligenes F-111, which was isolated from soil using a rhodamine B agar plate with Na2CO3. For enzyme production, olive oil, soymeal, triton X-100 and sodium ion were found to be essential. The addition of triton X-100 increased the alkaline lipase by 50- fold. The effect of additives as well as the development of suitable culture conditions for lipase production is discussed.

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Authors and Affiliations

  1. Department of Bioengineering, Tatung Institute of Technology, Taipei, Taiwan, Republic of China

    Shuen-Fuh Lin & Chien-Ming Chiou

  2. Institute of Biochemistry, National Yang-Ming University, Taipei, Taiwan, Republic of China

    Ying-Chieh Tsai

Authors
  1. Shuen-Fuh Lin
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  2. Chien-Ming Chiou
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  3. Ying-Chieh Tsai
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Lin, SF., Chiou, CM. & Tsai, YC. Effect of triton X-100 on alkaline lipase production by Pseudomonas pseudoalcaligenes F-111. Biotechnol Lett 17, 959–962 (1995). https://doi.org/10.1007/BF00127434

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  • DOI: https://doi.org/10.1007/BF00127434

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